Identification and Evolutionary Analysis of the Auxin Response Factor (ARF) Family Based on Transcriptome Data from Caucasian Clover and Analysis of Expression Responses to Hormones
Abstract
:1. Introduction
2. Results
2.1. Transcriptome-Wide Identification of ARFs in Caucasian Clover
2.2. Phylogenetic Analysis of TaARFs
2.3. Transcriptome-Wide Identification of ARFs in Caucasian Clover
2.4. Expression Patterns of TaARF Genes in Different Tissues of the Caucasian Clover Rhizome
2.5. Expression Analysis of TaARF Genes in Response to Hormones
3. Discussion
4. Materials and Methods
4.1. Identification and Sequence Analysis of ARF Genes from Caucasian Clover
4.2. Physicochemical Properties and Conserved Motif Analysis
4.3. Multiple Sequence Alignment and Phylogenetic Analysis
4.4. Expression Analysis of Different Tissue Sites
4.5. Plant Materials
4.6. RNA Extraction, cDNA Synthesis and Quantitative RT–PCR
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jiang, J.; Wang, Z.; Chen, Z.; Wu, Y.; Mu, M.; Nie, W.; Zhao, S.; Cui, G.; Yin, X. Identification and Evolutionary Analysis of the Auxin Response Factor (ARF) Family Based on Transcriptome Data from Caucasian Clover and Analysis of Expression Responses to Hormones. Int. J. Mol. Sci. 2023, 24, 15357. https://doi.org/10.3390/ijms242015357
Jiang J, Wang Z, Chen Z, Wu Y, Mu M, Nie W, Zhao S, Cui G, Yin X. Identification and Evolutionary Analysis of the Auxin Response Factor (ARF) Family Based on Transcriptome Data from Caucasian Clover and Analysis of Expression Responses to Hormones. International Journal of Molecular Sciences. 2023; 24(20):15357. https://doi.org/10.3390/ijms242015357
Chicago/Turabian StyleJiang, Jingwen, Zicheng Wang, Zirui Chen, Yuchen Wu, Meiqi Mu, Wanting Nie, Siwen Zhao, Guowen Cui, and Xiujie Yin. 2023. "Identification and Evolutionary Analysis of the Auxin Response Factor (ARF) Family Based on Transcriptome Data from Caucasian Clover and Analysis of Expression Responses to Hormones" International Journal of Molecular Sciences 24, no. 20: 15357. https://doi.org/10.3390/ijms242015357