CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing
Abstract
:1. Introduction
2. Results
2.1. Examining Immunological Cells within scRNA-Seq Samples
2.2. Infiltrating Immune Cell Count
2.3. The WGCNA Structure and Identification of Essential Modules
2.4. Analysis of the Relationship between Key Genes and Prognosis in Gastric Cancer
2.5. Screening the Key Genes with LASSO
2.6. GEPIA Database Analysis
2.7. TISIDB Database Verification of the Association between Key Genes and Immune Cell Invasion
2.8. Gene Set Enrichment Analysis
2.9. CXCR4 Is Overexpressed in STAD
2.10. CXCR4 Expression Correlates Positively with TIBs in Clinical Samples of STAD
3. Discussion
4. Materials and Methods
4.1. Expression Profile Information on Genes
4.2. Clustering Dimension Reduction for scRNA Seq Data and Immune Cell Screening
4.3. Infiltration of Immune Cells
4.4. Weighted Gene Coexpression Network Analysis (WGCNA)
4.5. Analysis for Univariate Cox Regression
4.6. Screening Key Genes via LASSO
4.7. Prognosis Database Analysis
4.8. TISIDB Database Verification of the Association between Essential Genes and Immune Cell Infiltration
4.9. Gene Set Enrichment Analysis
4.10. Clinical Samples
4.11. Extraction of RNA via RT-PCR
4.12. IHC Staining
4.13. Analyses of Statistics
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Clinicopathological Parameters | Expression | p-Value | |
---|---|---|---|
High | Low | ||
Age, years | |||
≤60 | 8 | 1 | 0.374 |
>60 | 14 | 7 | |
Gender | |||
Male | 17 | 7 | 0.655 |
Female | 5 | 1 | |
Location | |||
Cardiac | 3 | 5 | 0.024 |
Antrum | 6 | 1 | |
Pylorus | 13 | 2 | |
Tumor size | |||
≤5 cm | 16 | 4 | 0.384 |
>5 cm | 6 | 4 | |
Pathology grade | |||
II | 9 | 3 | 1 |
III | 13 | 5 | |
TNM stage | |||
I + II | 12 | 1 | 0.024 |
III + IV | 10 | 9 |
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Su, C.; Yu, R.; Hong, X.; Zhang, P.; Guo, Y.; Cai, J.-C.; Hou, J. CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing. Int. J. Mol. Sci. 2023, 24, 12890. https://doi.org/10.3390/ijms241612890
Su C, Yu R, Hong X, Zhang P, Guo Y, Cai J-C, Hou J. CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing. International Journal of Molecular Sciences. 2023; 24(16):12890. https://doi.org/10.3390/ijms241612890
Chicago/Turabian StyleSu, Chen, Rong Yu, Xiaoquan Hong, Panpan Zhang, Yingying Guo, Jian-Chun Cai, and Jingjing Hou. 2023. "CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing" International Journal of Molecular Sciences 24, no. 16: 12890. https://doi.org/10.3390/ijms241612890