Next Article in Journal
Resistance Is Not Futile: Widespread Convergent Evolution of Resistance to Alpha-Neurotoxic Snake Venoms in Caecilians (Amphibia: Gymnophiona)
Next Article in Special Issue
MEF2C and miR-194-5p: New Players in Triple Negative Breast Cancer Tumorigenesis
Previous Article in Journal
Artificial Intelligence in Decrypting Cytoprotective Activity under Oxidative Stress from Molecular Structure
Previous Article in Special Issue
Tamoxifen Modulates the Immune Landscape of the Tumour Microenvironment: The Paired Siglec-5/14 Checkpoint in Anti-Tumour Immunity in an In Vitro Model of Breast Cancer
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 24
Issue 14
10.3390/ijms241411352
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

The Role of ARHGAP1 in Rho GTPase Inactivation during Metastasizing of Breast Cancer Cell Line MCF-7 after Treatment with Doxorubicin

by
Imrich Géci
1,*,
Peter Bober
1,
Eva Filová
2,
Evžen Amler
2 and
Ján Sabo
1
1
Department of Medical and Clinical Biophysics, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 04011 Košice, Slovakia
2
Institute of Experimental Medicine, Czech Academy of Sciences, Vídeňská 1083, 142 00 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(14), 11352; https://doi.org/10.3390/ijms241411352
Submission received: 3 June 2023 / Revised: 5 July 2023 / Accepted: 8 July 2023 / Published: 12 July 2023
(This article belongs to the Special Issue Molecular Biology of Breast Cancer)
Browse Figures
Versions Notes

Abstract

Breast cancer is the most prevalent cancer type in women worldwide. It proliferates rapidly and can metastasize into farther tissues at any stage due to the gradual invasiveness and motility of the tumor cells. These crucial properties are the outcome of the weakened intercellular adhesion, regulated by small guanosine triphosphatases (GTPases), which hydrolyze to the guanosine diphosphate (GDP)-bound conformation. We investigated the inactivating effect of ARHGAP1 on Rho GTPases involved signaling pathways after treatment with a high dose of doxorubicin. Label-free quantitative proteomic analysis of the proteome isolated from the MCF-7 breast cancer cell line, treated with 1 μM of doxorubicin, identified RAC1, CDC42, and RHOA GTPases that were inactivated by the ARHGAP1 protein. Upregulation of the GTPases involved in the transforming growth factor-beta (TGF-beta) signaling pathway initiated epithelial–mesenchymal transitions. These findings demonstrate a key role of the ARHGAP1 protein in the disruption of the cell adhesion and simultaneously allow for a better understanding of the molecular mechanism of the reduced cell adhesion leading to the subsequent metastasis. The conclusions of this study corroborate the hypothesis that chemotherapy with doxorubicin may increase the risk of metastases in drug-resistant breast cancer cells.
Keywords: proteomics; mass spectrometry; cell adhesion; breast cancer; doxorubicin; metastases proteomics; mass spectrometry; cell adhesion; breast cancer; doxorubicin; metastases
Graphical Abstract

Share and Cite

MDPI and ACS Style

Géci, I.; Bober, P.; Filová, E.; Amler, E.; Sabo, J. The Role of ARHGAP1 in Rho GTPase Inactivation during Metastasizing of Breast Cancer Cell Line MCF-7 after Treatment with Doxorubicin. Int. J. Mol. Sci. 2023, 24, 11352. https://doi.org/10.3390/ijms241411352

AMA Style

Géci I, Bober P, Filová E, Amler E, Sabo J. The Role of ARHGAP1 in Rho GTPase Inactivation during Metastasizing of Breast Cancer Cell Line MCF-7 after Treatment with Doxorubicin. International Journal of Molecular Sciences. 2023; 24(14):11352. https://doi.org/10.3390/ijms241411352

Chicago/Turabian Style

Géci, Imrich, Peter Bober, Eva Filová, Evžen Amler, and Ján Sabo. 2023. "The Role of ARHGAP1 in Rho GTPase Inactivation during Metastasizing of Breast Cancer Cell Line MCF-7 after Treatment with Doxorubicin" International Journal of Molecular Sciences 24, no. 14: 11352. https://doi.org/10.3390/ijms241411352

APA Style

Géci, I., Bober, P., Filová, E., Amler, E., & Sabo, J. (2023). The Role of ARHGAP1 in Rho GTPase Inactivation during Metastasizing of Breast Cancer Cell Line MCF-7 after Treatment with Doxorubicin. International Journal of Molecular Sciences, 24(14), 11352. https://doi.org/10.3390/ijms241411352

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop