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Article

Thermodynamic Signatures of Blood Plasma Proteome in Neurodegenerative Pathologies

by
Avgustina Danailova
1,
Svetla Todinova
1,
Lidia Gartcheva
2,
Desislava Bogdanova
3,
Elena Zlatareva
3,
Nikolay Kalaydzhiev
3,
Ivan Milanov
3,
Sashka Krumova
1 and
Stefka G. Taneva
1,*
1
Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, “Acad. G. Bonchev” Str. 21, 1113 Sofia, Bulgaria
2
National Specialized Hospital for Active Treatment of Haematological Diseases, “Plovdivsko pole” Str. 6, 1756 Sofia, Bulgaria
3
University Multiprofile Hospital for Active Treatment in Neurology and Psychiatry “St. Naum”, “Louben Roussev” Str. 1, 1113 Sofia, Bulgaria
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(1), 789; https://doi.org/10.3390/ijms24010789
Submission received: 17 November 2022 / Revised: 23 December 2022 / Accepted: 29 December 2022 / Published: 2 January 2023
(This article belongs to the Special Issue Biomarkers and Molecular Methodologies in Neurodegenerative Diseases Diagnostic)
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Abstract

Discovery of diagnostic biomarkers for age-related neurodegenerative pathologies (NDDs) is essential for accurate diagnosis, following disease progression and drug development. Blood plasma and blood cells are important peripheral sources for NDDs’ biomarkers that, although present in lower concentrations than in cerebrospinal fluid, would allow noninvasive diagnostics. To identify new biomarkers for Parkinson’s disease (PD) and amyotrophic lateral sclerosis (ALS), in this work we have evaluated the modifications in the thermodynamic behavior of blood plasma proteome exploring differential scanning calorimetry. The plasma thermodynamics reflected the complexity and heterogeneity of the two pathologies. The unfolding temperature of the most abundant plasma protein albumin and the weighted average center of the calorimetric profile appeared as the two thermodynamic signatures that reflected modifications of the plasma proteome, i.e., strong thermal stabilization of albumin and plasma proteins’ interaction network, related to both pathologies. Based on those two signatures, both PD and ALS patients were stratified in two sets, except several cases with thermodynamic parameters that strongly differed from those of the calorimetric sets. Along with modifications of the plasma thermodynamic behavior, we found altered globulin levels in all PD and ALS patients’ plasma (higher level of α- and β-globulin fractions and lower level of γ-globulin fraction than the respective reference values) employing capillary electrophoresis. The presented results reveal the potential of calorimetry to indirectly identify NDDs’ biomarkers in blood plasma.
Keywords: blood plasma biomarkers; Parkinson’s disease; amyotrophic lateral sclerosis; differential scanning calorimetry; thermal transitions; transition temperature; excess heat capacity; globulin fractions blood plasma biomarkers; Parkinson’s disease; amyotrophic lateral sclerosis; differential scanning calorimetry; thermal transitions; transition temperature; excess heat capacity; globulin fractions

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MDPI and ACS Style

Danailova, A.; Todinova, S.; Gartcheva, L.; Bogdanova, D.; Zlatareva, E.; Kalaydzhiev, N.; Milanov, I.; Krumova, S.; Taneva, S.G. Thermodynamic Signatures of Blood Plasma Proteome in Neurodegenerative Pathologies. Int. J. Mol. Sci. 2023, 24, 789. https://doi.org/10.3390/ijms24010789

AMA Style

Danailova A, Todinova S, Gartcheva L, Bogdanova D, Zlatareva E, Kalaydzhiev N, Milanov I, Krumova S, Taneva SG. Thermodynamic Signatures of Blood Plasma Proteome in Neurodegenerative Pathologies. International Journal of Molecular Sciences. 2023; 24(1):789. https://doi.org/10.3390/ijms24010789

Chicago/Turabian Style

Danailova, Avgustina, Svetla Todinova, Lidia Gartcheva, Desislava Bogdanova, Elena Zlatareva, Nikolay Kalaydzhiev, Ivan Milanov, Sashka Krumova, and Stefka G. Taneva. 2023. "Thermodynamic Signatures of Blood Plasma Proteome in Neurodegenerative Pathologies" International Journal of Molecular Sciences 24, no. 1: 789. https://doi.org/10.3390/ijms24010789

APA Style

Danailova, A., Todinova, S., Gartcheva, L., Bogdanova, D., Zlatareva, E., Kalaydzhiev, N., Milanov, I., Krumova, S., & Taneva, S. G. (2023). Thermodynamic Signatures of Blood Plasma Proteome in Neurodegenerative Pathologies. International Journal of Molecular Sciences, 24(1), 789. https://doi.org/10.3390/ijms24010789

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