Ameliorative Effect of Ethanolic Echinacea purpurea against Hyperthyroidism-Induced Oxidative Stress via AMRK and PPAR Signal Pathway Using Transcriptomics and Network Pharmacology Analysis
, Weican Liang 2 and Linyuan Wang 1,*
Round 1
Reviewer 1 Report
Good research has been conducted and presented by authors
Introduction may be condensed.
Conclusion may be extended
figures especially gel pictures may be incorporated in main text
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
1- It is suggested to illustrate the mechanism PPAR signalling pathway and AMRK signalling pathway, that were considered the crucial signal pathways of the antioxidative mechanism of EEP against hyperthyroidism-induced oxidative stress. At the end of introduction you can add it and improve the manuscript.
2-It is suggest to indicate the bioactive compounds in Figure 1. UPLC-Q-TOF-MS analysis of ethanolic extracts of Echinacea Purpurea. This is usually the standard way so that the audience will understand easily and clearly.
3-Please check all raw file. Some file i cannot able to open it. Please check it before submit the manuscript. I hugely appreciate you include all raw data as supplementary file that stengthen the evidence.
4- Some pictures are blurred. for examples
Figure 7. Significant modular analysis and validation based on the PPI network of antioxidative perturbation genes of EEP. (A)(B)
5-Can you make an effort the strengthen the result with better discussion by inclusion of all key reference.
6- The botanical name must always be italic format. Check your manuscript.
7-Please draw the flow diagram on Preparation of Ethanolic Echinacea purpurea Extracts.
8-Please explain grinding meth0dology of plants.
9-Please explain drying methodology of plant.
10-Please explain sieve methodology of cut plant.
11- Please explain percentage of extract obtain and calculations.
12-Please include the heading of instrumentation and provide all instrument used with source.
13- It is mandatory to cite the methodology of have adopted and/or modified.
14. The study involve the animal study so mandatory to adhere the ARRIVE guidelines. https://arriveguidelines.org/
15- Please download the ARRIVE CHECKLIST, Include it as supplementary file and add one statement that the manuscript adhare to ARRIVE GUIDELINES.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
