Chromatin-Remodelling ATPases ISWI and BRM Are Essential for Reproduction in the Destructive Pest Tuta absoluta
Abstract
:1. Introduction
2. Results
2.1. Chromatin-Remodelling Gene Cloning
2.2. TaISWI and TaBRM Characterisation
2.3. TaISWI and TaBRM Expression Patterns during Different Life Cycle Stages
2.4. Effects of dsRNA Injection on TaISWI and TaBRM Expression
2.5. Effects of dsRNA Injection on Ovarian Development and Fecundity
3. Discussion
4. Materials and Methods
4.1. Insect Rearing
4.2. RNA Isolation and cDNA Synthesis
4.3. Chromatin-Remodelling Gene Cloning
4.4. Gene Sequences Analysis and Phylogenetic Relationships
4.5. RT-qPCR
4.6. Double-Stranded RNA (dsRNA) Synthesis
4.7. dsRNA Injection and Detection
4.8. Post-RNAi Ovary Development and Fecundity
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Primer Name | Primer Sequence (5′ to 3′) |
---|---|---|
Primers for cDNA cloning | ||
BRM | BRM-F1 | AACCATCACGCTAACGC |
BRM-R1 | GCTGGAACATGAAGGGAT | |
BRM-F2 | ATCATCAAGTGCGACAT | |
BRM-R2 | CCGACAGGACTCTACCG | |
BRM-F3 | CGGAAGCCAACCTACT | |
BRM-R3 | CCTGCCCTTTCACTAA | |
ISWI | ISWI-F1 | GCTAATGTATTTGGATAATTT |
ISWI-R1 | AGTATGGCGAGTTTCCC | |
ISWI-F2 | CGTCTCAAATCTGAAGTAG | |
ISWI-R2 | CCCAATGTCTTTCTGTAGT | |
ISWI-F3 | CAGAAGTTGGAGAGCCTA | |
ISWI-R3 | TCTACCGCTCATAACCG | |
Primers for qPCR | ||
BRM | BRM-QF | CAACGGAAAACTCAAGGAATACCA |
BRM-QR | GCACCCAGTTTGATAGCGTACTG | |
ISWI | ISWI-QF | GCTGATGAGATGGGTCTGGGT |
ISWI-QR | CACACTGCTCTTAGGGAAGGACA | |
RpL5 | RpL5-QF | CAGTCGTCGAGCCAGCAACA |
RpL5-QR | TCCCGCATTGAAGGAGACCA | |
Primers for dsRNA synthesis | ||
BRM | BRM-DF | TAATACGACTCACTATAGGGGAACCATCACGCTAACG |
BRM-DR | TAATACGACTCACTATAGGGGGATTCGTGGACCGTA | |
ISWI | ISWI-DF | TAATACGACTCACTATAGGGTATGGATGTAGGGGACG |
ISWI-DR | TAATACGACTCACTATAGGGCCAGTTGGTTAGGGTTG |
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Ji, S.-X.; Wu, Q.-W.; Bi, S.-Y.; Wang, X.-D.; Zhang, G.-F.; Wan, F.-H.; Lü, Z.-C.; Liu, W.-X. Chromatin-Remodelling ATPases ISWI and BRM Are Essential for Reproduction in the Destructive Pest Tuta absoluta. Int. J. Mol. Sci. 2022, 23, 3267. https://doi.org/10.3390/ijms23063267
Ji S-X, Wu Q-W, Bi S-Y, Wang X-D, Zhang G-F, Wan F-H, Lü Z-C, Liu W-X. Chromatin-Remodelling ATPases ISWI and BRM Are Essential for Reproduction in the Destructive Pest Tuta absoluta. International Journal of Molecular Sciences. 2022; 23(6):3267. https://doi.org/10.3390/ijms23063267
Chicago/Turabian StyleJi, Shun-Xia, Qiang-Wen Wu, Si-Yan Bi, Xiao-Di Wang, Gui-Fen Zhang, Fang-Hao Wan, Zhi-Chuang Lü, and Wan-Xue Liu. 2022. "Chromatin-Remodelling ATPases ISWI and BRM Are Essential for Reproduction in the Destructive Pest Tuta absoluta" International Journal of Molecular Sciences 23, no. 6: 3267. https://doi.org/10.3390/ijms23063267