Neuroprotection of Kaji-Ichigoside F1 via the BDNF/Akt/mTOR Signaling Pathways against NMDA-Induced Neurotoxicity
Abstract
:1. Introduction
2. Results
2.1. The Neuroprotective Mechanism of KF1 in PC12 Cells
2.1.1. KF1 Alleviated NMDA-Induced Neurotoxicity in PC12 Cells
2.1.2. Effects of KF1 on NMDA-Induced Ca2+ Overload
2.1.3. Effects of KF1 on NMDA-Induced Apoptosis in PC12 Cells
2.1.4. KF1 Effects on the Expression of NR2B, AMPA, BDNF, AKT, mTOR, PSD95, and Synapsin 1 (Syn1) Proteins in NMDA-Induced PC12 Cells by Immunofluorescence
2.2. In Vivo Analysis of the Mechanisms of Action of KF1
2.2.1. Effect of KF1 on Spatial Memory in the Y-Maze Test
2.2.2. Effect of KF1 on the Morris Water Maze (MWM) Test
2.2.3. Effects of KF1 on Neurotransmitters and Monoamine Oxidase (MAO) Levels in the Hippocampus
2.2.4. Effects of KF1 on Ca2+ Concentration
2.2.5. Histopathological Changes in the Hippocampus
2.2.6. Effects of KF1 on Hippocampal Neurons of NMDA-Induced Mice
2.2.7. Immunohistochemical Expression of NMDAR1 in the Hippocampus and Cortex
2.2.8. Western Blot Assay to Verify the Expression of NMDAR1, AMPA, BDNF, TrkB, AKT, mTOR, PSD95, and Syn1 In Vivo and In Vitro
3. Discussion
4. Materials and Methods
4.1. In Vitro Analysis of the Mechanisms of Action of KF1
4.1.1. Cell Management and Viability
4.1.2. LDH Release Assay
4.1.3. Measurement of Intracellular Ca2+ Concentration
4.1.4. Hoechst 33342 Staining
4.1.5. Annexin V-FITC/PI Flow Cytometry Assay
4.1.6. Fluorescence Staining
4.2. In Vivo Analysis of the Mechanisms of Action of KF1
4.2.1. Animals
4.2.2. Experimental Designs
4.2.3. Y-Maze Test
4.2.4. Morris Water Maze Test
4.2.5. Quantification of 5-HT, DA, MAO, and Ca2+ Concentration in the Hippocampus by ELISA
4.2.6. HE Staining of the Hippocampus
4.2.7. Nissl Staining of the Hippocampus
4.2.8. Immunohistochemistry Assay for NMDAR1 in the Hippocampus and Cortex
4.2.9. Western Blot
4.3. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Chen, F.; Wang, L.; Jin, F.; Li, L.; Wang, T.; Gao, M.; Li, L.; Wang, Y.; Lou, Z.; Yang, J.; et al. Neuroprotection of Kaji-Ichigoside F1 via the BDNF/Akt/mTOR Signaling Pathways against NMDA-Induced Neurotoxicity. Int. J. Mol. Sci. 2022, 23, 16150. https://doi.org/10.3390/ijms232416150
Chen F, Wang L, Jin F, Li L, Wang T, Gao M, Li L, Wang Y, Lou Z, Yang J, et al. Neuroprotection of Kaji-Ichigoside F1 via the BDNF/Akt/mTOR Signaling Pathways against NMDA-Induced Neurotoxicity. International Journal of Molecular Sciences. 2022; 23(24):16150. https://doi.org/10.3390/ijms232416150
Chicago/Turabian StyleChen, Faju, Li Wang, Fengli Jin, Liangqun Li, Tao Wang, Ming Gao, Lilang Li, Yu Wang, Zhongsheng Lou, Juan Yang, and et al. 2022. "Neuroprotection of Kaji-Ichigoside F1 via the BDNF/Akt/mTOR Signaling Pathways against NMDA-Induced Neurotoxicity" International Journal of Molecular Sciences 23, no. 24: 16150. https://doi.org/10.3390/ijms232416150