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Article

Isolation and Characterization of Two Novel Siphoviruses Novomoskovsk and Bolokhovo, Encoding Polysaccharide Depolymerases Active against Bacillus pumilus

by
Anna V. Skorynina
1,*,
Olga N. Koposova
1,
Olesya A. Kazantseva
1,
Emma G. Piligrimova
1,
Natalya A. Ryabova
2 and
Andrey M. Shadrin
1,*
1
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Federal Research Center, Prospect Nauki, 5, 142290 Pushchino, Russia
2
Institute of Protein Research RAS, Institutskaya st., 4, 142290 Pushchino, Russia
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(21), 12988; https://doi.org/10.3390/ijms232112988
Submission received: 31 July 2022 / Revised: 5 October 2022 / Accepted: 22 October 2022 / Published: 26 October 2022
(This article belongs to the Special Issue Bacteriophage Biology: From Genomics to Therapy)
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Abstract

This study describes two novel bacteriophages infecting members of the Bacillus pumilus group. Even though members of the group are not recognized as pathogenic, several strains belonging to the group have been reported to cause infectious diseases in plants, animals and humans. Bacillus pumilus group species are highly resistant to ultraviolet radiation and capable of forming biofilms, which complicates their eradication. Bacteriophages Novomoskovsk and Bolokhovo were isolated from soil samples. Genome sequencing and phylogenetic analysis revealed that the phages represent two new species of the genus Andromedavirus (class Caudoviricetes). The phages remained stable in a wide range of temperatures and pH values. A host range test showed that the phages specifically infect various strains of B. pumilus. The phages form clear plaques surrounded by halos. Both phages Novomoskovsk and Bolokhovo encode proteins with pectin lyase domains—Putative depolymerases. Obtained in a purified recombinant form, the proteins produced lysis zones on the lawn of a B. pumilus strain. This suggests that Novomoskovsk and Bolokhovo may be effective for the eradication of B. pumilus biofilms.
Keywords: bacteriophage; Bacillus pumilus; Andromedavirus; depolymerase; biocontrol; genome sequencing bacteriophage; Bacillus pumilus; Andromedavirus; depolymerase; biocontrol; genome sequencing

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MDPI and ACS Style

Skorynina, A.V.; Koposova, O.N.; Kazantseva, O.A.; Piligrimova, E.G.; Ryabova, N.A.; Shadrin, A.M. Isolation and Characterization of Two Novel Siphoviruses Novomoskovsk and Bolokhovo, Encoding Polysaccharide Depolymerases Active against Bacillus pumilus. Int. J. Mol. Sci. 2022, 23, 12988. https://doi.org/10.3390/ijms232112988

AMA Style

Skorynina AV, Koposova ON, Kazantseva OA, Piligrimova EG, Ryabova NA, Shadrin AM. Isolation and Characterization of Two Novel Siphoviruses Novomoskovsk and Bolokhovo, Encoding Polysaccharide Depolymerases Active against Bacillus pumilus. International Journal of Molecular Sciences. 2022; 23(21):12988. https://doi.org/10.3390/ijms232112988

Chicago/Turabian Style

Skorynina, Anna V., Olga N. Koposova, Olesya A. Kazantseva, Emma G. Piligrimova, Natalya A. Ryabova, and Andrey M. Shadrin. 2022. "Isolation and Characterization of Two Novel Siphoviruses Novomoskovsk and Bolokhovo, Encoding Polysaccharide Depolymerases Active against Bacillus pumilus" International Journal of Molecular Sciences 23, no. 21: 12988. https://doi.org/10.3390/ijms232112988

APA Style

Skorynina, A. V., Koposova, O. N., Kazantseva, O. A., Piligrimova, E. G., Ryabova, N. A., & Shadrin, A. M. (2022). Isolation and Characterization of Two Novel Siphoviruses Novomoskovsk and Bolokhovo, Encoding Polysaccharide Depolymerases Active against Bacillus pumilus. International Journal of Molecular Sciences, 23(21), 12988. https://doi.org/10.3390/ijms232112988

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