Overexpression of Sweet Potato Carotenoid Cleavage Dioxygenase 4 (IbCCD4) Decreased Salt Tolerance in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Bioinformatics Analysis of IbCCD4
2.2. IbCCD4 Response to Salt and Dehydration Stress
2.3. The Ectopic Expression of IbCCD4 Reduced Salt Tolerance in Transgenic Arabidopsis
2.4. The Ectopic Expression of IbCCD4 Increased the Total Carotenoid Content and the Expression Levels of Most Carotenoid Biosynthesis Genes in Transgenic Arabidopsis
2.5. The Ectopic Expression of IbCCD4 Decreased the Expression of Resistance-Related Genes in Transgenic Arabidopsis under Salt Stress
2.6. The Ectopic Expression of IbCCD4 Decreased Antioxidant Enzyme Activity in Transgenic Arabidopsis under Salt Stress
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. IbCCD4 Multiple Sequence Alignment, Phylogenetic Tree Construction, and Motif Analysis
4.3. Salt and Dehydration Stress Treatment
4.4. Total RNA Extraction, cDNA Synthesis, and Expression Analysis by qRT-PCR
4.5. Cloning of the IbCCD4 Genomic Sequence and Gene Structure Analysis
4.6. Generation of Transgenic Arabidopsis
4.7. Salt Stress Assay of IbCCD4-Overexpressing Arabidopsis
4.8. Statistical Analysis of Data
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhang, J.; He, L.; Dong, J.; Zhao, C.; Tang, R.; Jia, X. Overexpression of Sweet Potato Carotenoid Cleavage Dioxygenase 4 (IbCCD4) Decreased Salt Tolerance in Arabidopsis thaliana. Int. J. Mol. Sci. 2022, 23, 9963. https://doi.org/10.3390/ijms23179963
Zhang J, He L, Dong J, Zhao C, Tang R, Jia X. Overexpression of Sweet Potato Carotenoid Cleavage Dioxygenase 4 (IbCCD4) Decreased Salt Tolerance in Arabidopsis thaliana. International Journal of Molecular Sciences. 2022; 23(17):9963. https://doi.org/10.3390/ijms23179963
Chicago/Turabian StyleZhang, Jie, Liheng He, Jingjing Dong, Cailiang Zhao, Ruimin Tang, and Xiaoyun Jia. 2022. "Overexpression of Sweet Potato Carotenoid Cleavage Dioxygenase 4 (IbCCD4) Decreased Salt Tolerance in Arabidopsis thaliana" International Journal of Molecular Sciences 23, no. 17: 9963. https://doi.org/10.3390/ijms23179963