Cultured Myoblasts Derived from Rat Soleus Muscle Show Altered Regulation of Proliferation and Myogenesis during the Course of Mechanical Unloading

Komarova, Margarita Y.; Rozhkov, Sergey V.; Ivanova, Oksana A.; Turtikova, Olga V.; Mirzoev, Timur M.; Dmitrieva, Renata I.; Shenkman, Boris S.; Vilchinskaya, Natalia A.

doi:10.3390/ijms23169150

Open AccessArticle

Cultured Myoblasts Derived from Rat Soleus Muscle Show Altered Regulation of Proliferation and Myogenesis during the Course of Mechanical Unloading

by

Margarita Y. Komarova

^1,2

,

Sergey V. Rozhkov

¹,

Oksana A. Ivanova

²

,

Olga V. Turtikova

¹

,

Timur M. Mirzoev

¹

,

Renata I. Dmitrieva

^2,*

,

Boris S. Shenkman

¹ and

Natalia A. Vilchinskaya

^1,*

¹

Myology Laboratory, Institute of Biomedical Problems RAS, 123007 Moscow, Russia

²

Institute of Molecular Biology and Genetics, Almazov National Medical Research Centre, 197341 Saint Petersburg, Russia

^*

Authors to whom correspondence should be addressed.

Int. J. Mol. Sci. 2022, 23(16), 9150; https://doi.org/10.3390/ijms23169150

Submission received: 1 July 2022 / Revised: 4 August 2022 / Accepted: 10 August 2022 / Published: 15 August 2022

(This article belongs to the Special Issue Myoblast and Muscle Cell Genesis and Regeneration)

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Abstract

The structure and function of soleus muscle fibers undergo substantial remodeling under real or simulated microgravity conditions. However, unloading-induced changes in the functional activity of skeletal muscle primary myoblasts remain poorly studied. The purpose of our study was to investigate how short-term and long-term mechanical unloading would affect cultured myoblasts derived from rat soleus muscle. Mechanical unloading was simulated by rat hindlimb suspension model (HS). Myoblasts were purified from rat soleus at basal conditions and after 1, 3, 7, and 14 days of HS. Myoblasts were expanded in vitro, and the myogenic nature was confirmed by their ability to differentiate as well as by immunostaining/mRNA expression of myogenic markers. The proliferation activity at different time points after HS was analyzed, and transcriptome analysis was performed. We have shown that soleus-derived myoblasts differently respond to an early and later stage of HS. At the early stage of HS, the proliferative activity of myoblasts was slightly decreased, and processes related to myogenesis activation were downregulated. At the later stage of HS, we observed a decrease in myoblast proliferative potential and spontaneous upregulation of the pro-myogenic program.

Keywords: soleus muscle; mechanical unloading; myoblast; MRF; proliferation; differentiation; myogenesis; transcriptome analysis

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MDPI and ACS Style

Komarova, M.Y.; Rozhkov, S.V.; Ivanova, O.A.; Turtikova, O.V.; Mirzoev, T.M.; Dmitrieva, R.I.; Shenkman, B.S.; Vilchinskaya, N.A. Cultured Myoblasts Derived from Rat Soleus Muscle Show Altered Regulation of Proliferation and Myogenesis during the Course of Mechanical Unloading. Int. J. Mol. Sci. 2022, 23, 9150. https://doi.org/10.3390/ijms23169150

AMA Style

Komarova MY, Rozhkov SV, Ivanova OA, Turtikova OV, Mirzoev TM, Dmitrieva RI, Shenkman BS, Vilchinskaya NA. Cultured Myoblasts Derived from Rat Soleus Muscle Show Altered Regulation of Proliferation and Myogenesis during the Course of Mechanical Unloading. International Journal of Molecular Sciences. 2022; 23(16):9150. https://doi.org/10.3390/ijms23169150

Chicago/Turabian Style

Komarova, Margarita Y., Sergey V. Rozhkov, Oksana A. Ivanova, Olga V. Turtikova, Timur M. Mirzoev, Renata I. Dmitrieva, Boris S. Shenkman, and Natalia A. Vilchinskaya. 2022. "Cultured Myoblasts Derived from Rat Soleus Muscle Show Altered Regulation of Proliferation and Myogenesis during the Course of Mechanical Unloading" International Journal of Molecular Sciences 23, no. 16: 9150. https://doi.org/10.3390/ijms23169150

APA Style

Komarova, M. Y., Rozhkov, S. V., Ivanova, O. A., Turtikova, O. V., Mirzoev, T. M., Dmitrieva, R. I., Shenkman, B. S., & Vilchinskaya, N. A. (2022). Cultured Myoblasts Derived from Rat Soleus Muscle Show Altered Regulation of Proliferation and Myogenesis during the Course of Mechanical Unloading. International Journal of Molecular Sciences, 23(16), 9150. https://doi.org/10.3390/ijms23169150

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Cultured Myoblasts Derived from Rat Soleus Muscle Show Altered Regulation of Proliferation and Myogenesis during the Course of Mechanical Unloading

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