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Volume 23
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Article

Fluctuations in Corneal Endothelial LAP2 Expression Levels Correlate with Passage Dependent Declines in Their Cell Proliferative Activity

by
Eleonora Maurizi
1,*,
Alessia Merra
2,
Davide Schiroli
3,
Benedetta Ghezzi
4,
Claudio Macaluso
4 and
Graziella Pellegrini
1,2
1
Centre for Regenerative Medicine, University of Modena and Reggio Emilia, 41125 Modena, Italy
2
Holostem Terapie Avanzate S.r.l., 41125 Modena, Italy
3
Transfusion Medicine Unit, Azienda USL-IRCCS, 42123 Reggio Emilia, Italy
4
Dentistry Centre Lab, University of Parma, 43126 Parma, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(10), 5859; https://doi.org/10.3390/ijms23105859
Submission received: 2 May 2022 / Revised: 19 May 2022 / Accepted: 22 May 2022 / Published: 23 May 2022
(This article belongs to the Special Issue Stem Cell Biology & Regenerative Medicine)
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Abstract

The corneal endothelium is the inner corneal mono-layered epithelium, fundamental for preserving corneal hydration and transparency. However, molecular mechanisms that regulate corneal endothelial cells (CEnCs), in particular regarding their proliferative capacity, have been only partially elucidated. CEnCs are quiescent in vivo and they easily undergo endothelial to mesenchymal transition (EnMT) in vitro. This study aims to analyze CEnCs behavior and expression in vitro, either in sub-confluent growing (S) or confluent (C) CEnCs cultures. Primary rabbit and human CEnCs were cultured and used for RT-PCR, immunofluorescence or western blot analysis. These methods allowed identifying a novel molecular marker, LAP2, that is upregulated in S while downregulated in C human or rabbit CEnCs. Those results were observed for several subsequent passages in culture and this, together with the correlation between ki67 and LAP2 expression, suggested LAP2 as a novel possible indicator for culture ageing. Finally, treatment with FGF and TGFβ in rCEnCs highlighted how LAP2 can vary as the cells regulate their proliferative state. In conclusion, we have identified a novel marker for CEnCs, LAP2, that regulates its expression depending on the cells sub/confluent state and that correlates with CEnCs proliferation.
Keywords: LAP2; corneal endothelium; fluctuating; marker; confluent; proliferation LAP2; corneal endothelium; fluctuating; marker; confluent; proliferation

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MDPI and ACS Style

Maurizi, E.; Merra, A.; Schiroli, D.; Ghezzi, B.; Macaluso, C.; Pellegrini, G. Fluctuations in Corneal Endothelial LAP2 Expression Levels Correlate with Passage Dependent Declines in Their Cell Proliferative Activity. Int. J. Mol. Sci. 2022, 23, 5859. https://doi.org/10.3390/ijms23105859

AMA Style

Maurizi E, Merra A, Schiroli D, Ghezzi B, Macaluso C, Pellegrini G. Fluctuations in Corneal Endothelial LAP2 Expression Levels Correlate with Passage Dependent Declines in Their Cell Proliferative Activity. International Journal of Molecular Sciences. 2022; 23(10):5859. https://doi.org/10.3390/ijms23105859

Chicago/Turabian Style

Maurizi, Eleonora, Alessia Merra, Davide Schiroli, Benedetta Ghezzi, Claudio Macaluso, and Graziella Pellegrini. 2022. "Fluctuations in Corneal Endothelial LAP2 Expression Levels Correlate with Passage Dependent Declines in Their Cell Proliferative Activity" International Journal of Molecular Sciences 23, no. 10: 5859. https://doi.org/10.3390/ijms23105859

APA Style

Maurizi, E., Merra, A., Schiroli, D., Ghezzi, B., Macaluso, C., & Pellegrini, G. (2022). Fluctuations in Corneal Endothelial LAP2 Expression Levels Correlate with Passage Dependent Declines in Their Cell Proliferative Activity. International Journal of Molecular Sciences, 23(10), 5859. https://doi.org/10.3390/ijms23105859

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