Dynamics of Connexin 43 Down Modulation in Human Articular Chondrocytes Stimulated by Tumor Necrosis Factor Alpha
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. Cell Cultures
3.2. Treatments
3.3. Cell Lysates
3.4. Subcellular Fractionation
3.5. Western Blotting
3.6. Gene Expression Analysis
3.7. Scrape Loading Assay
3.8. Immunofluorescence
3.9. nLC-MS/MS Analysis for Protein Identification
3.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Della Morte, E.; Niada, S.; Giannasi, C.; Zagra, L.; Brini, A.T. Dynamics of Connexin 43 Down Modulation in Human Articular Chondrocytes Stimulated by Tumor Necrosis Factor Alpha. Int. J. Mol. Sci. 2022, 23, 5575. https://doi.org/10.3390/ijms23105575
Della Morte E, Niada S, Giannasi C, Zagra L, Brini AT. Dynamics of Connexin 43 Down Modulation in Human Articular Chondrocytes Stimulated by Tumor Necrosis Factor Alpha. International Journal of Molecular Sciences. 2022; 23(10):5575. https://doi.org/10.3390/ijms23105575
Chicago/Turabian StyleDella Morte, Elena, Stefania Niada, Chiara Giannasi, Luigi Zagra, and Anna Teresa Brini. 2022. "Dynamics of Connexin 43 Down Modulation in Human Articular Chondrocytes Stimulated by Tumor Necrosis Factor Alpha" International Journal of Molecular Sciences 23, no. 10: 5575. https://doi.org/10.3390/ijms23105575