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Open AccessArticle

Composites of Nucleic Acids and Boron Clusters (C2B10H12) as Functional Nanoparticles for Downregulation of EGFR Oncogene in Cancer Cells

1
Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, Poland
2
Department of Inorganic, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, Krzywoustego 6, 44-100 Gliwice, Poland
3
Laboratory of Medicinal Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 92-232 Lodz, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Vincenza Barresi
Int. J. Mol. Sci. 2021, 22(9), 4863; https://doi.org/10.3390/ijms22094863
Received: 18 March 2021 / Revised: 27 April 2021 / Accepted: 30 April 2021 / Published: 4 May 2021
Epidermal growth factor receptor (EGFR) is one of the most promising molecular targets for anticancer therapy. We used boron clusters as a platform for generation of new materials. For this, functional DNA constructs conjugated with boron clusters (B-ASOs) were developed. These B-ASOs, built from 1,2-dicarba-closo-dodecaborane linked with two anti-EGFR antisense oligonucleotides (ASOs), form with their complementary congeners torus-like nanostructures, as previously shown by atomic force microscope (AFM) and transmission electron cryo-microscopy (cryo-TEM) imaging. In the present work, deepened studies were carried out on B-ASO’s properties. In solution, B-ASOs formed four dominant complexes as confirmed by non-denaturing polyacrylamide gel electrophoresis (PAGE). These complexes exhibited increased stability in cell lysate comparing to the non-modified ASO. Fluorescently labeled B-ASOs localized mostly in the cytoplasm and decreased EGFR expression by activating RNase H. Moreover, the B-ASO complexes altered the cancer cell phenotype, decreased cell migration rate, and arrested the cells in the S phase of cell cycle. The 1,2-dicarba-closo-dodecaborane-containing nanostructures did not activate NLRP3 inflammasome in human macrophages. In addition, as shown by inductively coupled plasma mass spectrometry (ICP MS), these nanostructures effectively penetrated the human squamous carcinoma cells (A431), showing their potential applicability as anticancer agents. View Full-Text
Keywords: antisense oligonucleotide; boron cluster; nanostructure; EGFR; macrophages; cellular uptake antisense oligonucleotide; boron cluster; nanostructure; EGFR; macrophages; cellular uptake
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MDPI and ACS Style

Kaniowski, D.; Ebenryter-Olbińska, K.; Kulik, K.; Suwara, J.; Cypryk, W.; Jakóbik-Kolon, A.; Leśnikowski, Z.; Nawrot, B. Composites of Nucleic Acids and Boron Clusters (C2B10H12) as Functional Nanoparticles for Downregulation of EGFR Oncogene in Cancer Cells. Int. J. Mol. Sci. 2021, 22, 4863. https://doi.org/10.3390/ijms22094863

AMA Style

Kaniowski D, Ebenryter-Olbińska K, Kulik K, Suwara J, Cypryk W, Jakóbik-Kolon A, Leśnikowski Z, Nawrot B. Composites of Nucleic Acids and Boron Clusters (C2B10H12) as Functional Nanoparticles for Downregulation of EGFR Oncogene in Cancer Cells. International Journal of Molecular Sciences. 2021; 22(9):4863. https://doi.org/10.3390/ijms22094863

Chicago/Turabian Style

Kaniowski, Damian; Ebenryter-Olbińska, Katarzyna; Kulik, Katarzyna; Suwara, Justyna; Cypryk, Wojciech; Jakóbik-Kolon, Agata; Leśnikowski, Zbigniew; Nawrot, Barbara. 2021. "Composites of Nucleic Acids and Boron Clusters (C2B10H12) as Functional Nanoparticles for Downregulation of EGFR Oncogene in Cancer Cells" Int. J. Mol. Sci. 22, no. 9: 4863. https://doi.org/10.3390/ijms22094863

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