Next Article in Journal
CircRNA—Protein Interactions in Muscle Development and Diseases
Next Article in Special Issue
Trichodermin Induces G0/G1 Cell Cycle Arrest by Inhibiting c-Myc in Ovarian Cancer Cells and Tumor Xenograft-Bearing Mice
Previous Article in Journal
Behavioral Alterations and Decreased Number of Parvalbumin-Positive Interneurons in Wistar Rats after Maternal Immune Activation by Lipopolysaccharide: Sex Matters
Previous Article in Special Issue
Natural Health Products (NHP’s) and Natural Compounds as Therapeutic Agents for the Treatment of Cancer; Mechanisms of Anti-Cancer Activity of Natural Compounds and Overall Trends
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 22
Issue 6
10.3390/ijms22063273
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Regulatory Role of Sugars on the Settlement Inducing Activity of a Conspecific Cue in Pacific Oyster Crassostrea gigas

by
Mary Grace Sedanza
1,2,*,
Hee-Jin Kim
1,
Xerxes Seposo
3,
Asami Yoshida
1,
Kenichi Yamaguchi
1 and
Cyril Glenn Satuito
1,4
1
Graduate School of Fisheries and Environmental Sciences, Nagasaki University, Nagasaki 852-8521, Japan
2
Institute of Aquaculture, College of Fisheries and Ocean Sciences, University of the Philippines Visayas, Miagao, Iloilo city 5023, Philippines
3
School of Tropical Medicine and Global Health, Nagasaki University, Nagasaki 852-8523, Japan
4
Organization for Marine Science and Technology, Nagasaki University, Nagasaki 852-8521, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(6), 3273; https://doi.org/10.3390/ijms22063273
Submission received: 25 February 2021 / Revised: 15 March 2021 / Accepted: 20 March 2021 / Published: 23 March 2021
(This article belongs to the Special Issue Natural Compounds with Cancer-Selective Toxicity)
Browse Figures
Versions Notes

Abstract

This study evaluated the larval settlement inducing effect of sugars and a conspecific cue from adult shell extract of Crassostrea gigas. To understand how the presence of different chemical cues regulate settlement behavior, oyster larvae were exposed to 12 types of sugars, shell extract-coated and non-coated surfaces, and under varied sugar exposure times. Lectin-glycan interaction effects on settlement and its localization on oyster larval tissues were investigated. The results showed that the conspecific cue elicited a positive concentration dependent settlement inducing trend. Sugars in the absence of a conspecific cue, C. gigas adult shell extract, did not promote settlement. Whereas, in the presence of the cue, showed varied effects, most of which were found inhibitory at different concentrations. Sugar treated larvae exposed for 2 h showed significant settlement inhibition in the presence of a conspecific cue. Neu5Ac, as well as GlcNAc sugars, showed a similar interaction trend with wheat germ agglutinin (WGA) lectin. WGA-FITC conjugate showed positive binding on the foot, velum, and mantle when exposed to GlcNAc sugars. This study suggests that a WGA lectin-like receptor and its endogenous ligand are both found in the larval chemoreceptors and the shell Ethylenediaminetetraacetic acid (EDTA) extract that may complementarily work together to allow the oyster larva greater selectivity during site selection.
Keywords: Crassostrea gigas; Pacific oyster; conspecific cues; sugars; lectin; larval settlement Crassostrea gigas; Pacific oyster; conspecific cues; sugars; lectin; larval settlement

Share and Cite

MDPI and ACS Style

Sedanza, M.G.; Kim, H.-J.; Seposo, X.; Yoshida, A.; Yamaguchi, K.; Satuito, C.G. Regulatory Role of Sugars on the Settlement Inducing Activity of a Conspecific Cue in Pacific Oyster Crassostrea gigas. Int. J. Mol. Sci. 2021, 22, 3273. https://doi.org/10.3390/ijms22063273

AMA Style

Sedanza MG, Kim H-J, Seposo X, Yoshida A, Yamaguchi K, Satuito CG. Regulatory Role of Sugars on the Settlement Inducing Activity of a Conspecific Cue in Pacific Oyster Crassostrea gigas. International Journal of Molecular Sciences. 2021; 22(6):3273. https://doi.org/10.3390/ijms22063273

Chicago/Turabian Style

Sedanza, Mary Grace, Hee-Jin Kim, Xerxes Seposo, Asami Yoshida, Kenichi Yamaguchi, and Cyril Glenn Satuito. 2021. "Regulatory Role of Sugars on the Settlement Inducing Activity of a Conspecific Cue in Pacific Oyster Crassostrea gigas" International Journal of Molecular Sciences 22, no. 6: 3273. https://doi.org/10.3390/ijms22063273

APA Style

Sedanza, M. G., Kim, H.-J., Seposo, X., Yoshida, A., Yamaguchi, K., & Satuito, C. G. (2021). Regulatory Role of Sugars on the Settlement Inducing Activity of a Conspecific Cue in Pacific Oyster Crassostrea gigas. International Journal of Molecular Sciences, 22(6), 3273. https://doi.org/10.3390/ijms22063273

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop