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Article

Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in Drosophila

1
Department of Gene Expression Regulation in Development, Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, Russia
2
Center for Genetics and Life Science, Sirius University of Science and Technology, 354340 Sochi, Russia
3
Department of Biology and General Genetics, Sechenov First Moscow State Medical University (Sechenov University), 119992 Moscow, Russia
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Department of Biology, Università di Napoli Federico II, 80138 Naples, Italy
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Department of Sciences, Roma Tre University, 00154 Rome, Italy
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Institute of Research on Terrestrial Ecosystems (IRET) National Research Council (CNR), 05010 Porano, Italy
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Institut de Biologie Valrose iBV UMR CNRS 7277, Université Côte d’Azur, 06108 Nice, France
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Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Ctirad Hofr
Int. J. Mol. Sci. 2021, 22(6), 2856; https://doi.org/10.3390/ijms22062856
Received: 7 February 2021 / Revised: 3 March 2021 / Accepted: 8 March 2021 / Published: 11 March 2021
The chromatin remodeler SWI/SNF is an important participant in gene activation, functioning predominantly by opening the chromatin structure on promoters and enhancers. Here, we describe its novel mode of action in which SWI/SNF factors mediate the targeted action of an enhancer. We studied the functions of two signature subunits of PBAP subfamily, BAP170 and SAYP, in Drosophila. These subunits were stably tethered to a transgene reporter carrying the hsp70 core promoter. The tethered subunits mediate transcription of the reporter in a pattern that is generated by enhancers close to the insertion site in multiple loci throughout the genome. Both tethered SAYP and BAP170 recruit the whole PBAP complex to the reporter promoter. However, we found that BAP170-dependent transcription is more resistant to the depletion of other PBAP subunits, suggesting that BAP170 may play a more critical role in establishing enhancer-dependent transcription. View Full-Text
Keywords: SWI/SNF; PBAP; chromatin remodeling; promoter; enhancer SWI/SNF; PBAP; chromatin remodeling; promoter; enhancer
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MDPI and ACS Style

Shidlovskii, Y.V.; Bylino, O.V.; Shaposhnikov, A.V.; Kachaev, Z.M.; Lebedeva, L.A.; Kolesnik, V.V.; Amendola, D.; De Simone, G.; Formicola, N.; Schedl, P.; Digilio, F.A.; Giordano, E. Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in Drosophila. Int. J. Mol. Sci. 2021, 22, 2856. https://doi.org/10.3390/ijms22062856

AMA Style

Shidlovskii YV, Bylino OV, Shaposhnikov AV, Kachaev ZM, Lebedeva LA, Kolesnik VV, Amendola D, De Simone G, Formicola N, Schedl P, Digilio FA, Giordano E. Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in Drosophila. International Journal of Molecular Sciences. 2021; 22(6):2856. https://doi.org/10.3390/ijms22062856

Chicago/Turabian Style

Shidlovskii, Yulii V.; Bylino, Oleg V.; Shaposhnikov, Alexander V.; Kachaev, Zaur M.; Lebedeva, Lyubov A.; Kolesnik, Valeria V.; Amendola, Diego; De Simone, Giovanna; Formicola, Nadia; Schedl, Paul; Digilio, Filomena A.; Giordano, Ennio. 2021. "Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in Drosophila" Int. J. Mol. Sci. 22, no. 6: 2856. https://doi.org/10.3390/ijms22062856

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