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Article

Investigation of H2S Donor Treatment on Neutrophil Extracellular Traps in Experimental Colitis

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Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary
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Albert Szent-Györgyi Clinical Center, Department of Medicine, Medical Faculty, University of Szeged, H-6720 Szeged, Hungary
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Interdisciplinary Excellence Center, Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary
*
Author to whom correspondence should be addressed.
Academic Editors: Marcin Magierowski and Binghe Wang
Int. J. Mol. Sci. 2021, 22(23), 12729; https://doi.org/10.3390/ijms222312729
Received: 8 October 2021 / Revised: 18 November 2021 / Accepted: 22 November 2021 / Published: 25 November 2021
Inflammatory bowel diseases (IBD) are chronic, immune-mediated disorders, which affect the gastrointestinal tract with intermittent ulceration. It is increasingly clear that neutrophil extracellular traps (NETs) seem to have a role in IBD; however, the associated pathogenesis is still not known. Furthermore, several conventional therapies are available against IBD, although these might have side effects. Our current study aimed to investigate the effects of hydrogen sulfide (H2S) treatment on NETs formation and on the expression of inflammatory mediators in experimental rat colitis. To model IBD, 2,4,6-trinitrobenzenesulfonic acid (TNBS) was administered intracolonically (i.c.) to Wistar–Harlan male rats. Animals were treated (2 times/day) with H2S donor Lawesson’s reagent per os. Our results showed that H2S treatment significantly decreased the extent of colonic lesions. Furthermore, the expression of members of NETs formation: peptidyl arginine deiminase 4 (PAD4), citrullinated histone H3 (citH3), myeloperoxidase (MPO) and inflammatory regulators, such as nuclear transcription factor-kappa B (NF-κB) and high-mobility group box 1 (HMGB1) were reduced in H2S treated group compared to TNBS. Additionally, H2S donor administration elevated the expression of ubiquitin C-terminal hydroxylase L1 (UCHL-1), a potential anti-inflammatory mediator. Taken together, our results showed that H2S may exert anti-inflammatory effect through the inhibition of NETs formation, which suggests a new therapeutic approach against IBD. View Full-Text
Keywords: hydrogen sulfide; inflammation; inflammatory bowel disease; colitis; neutrophil extracellular traps hydrogen sulfide; inflammation; inflammatory bowel disease; colitis; neutrophil extracellular traps
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MDPI and ACS Style

Török, S.; Almási, N.; Valkusz, Z.; Pósa, A.; Varga, C.; Kupai, K. Investigation of H2S Donor Treatment on Neutrophil Extracellular Traps in Experimental Colitis. Int. J. Mol. Sci. 2021, 22, 12729. https://doi.org/10.3390/ijms222312729

AMA Style

Török S, Almási N, Valkusz Z, Pósa A, Varga C, Kupai K. Investigation of H2S Donor Treatment on Neutrophil Extracellular Traps in Experimental Colitis. International Journal of Molecular Sciences. 2021; 22(23):12729. https://doi.org/10.3390/ijms222312729

Chicago/Turabian Style

Török, Szilvia, Nikoletta Almási, Zsuzsanna Valkusz, Anikó Pósa, Csaba Varga, and Krisztina Kupai. 2021. "Investigation of H2S Donor Treatment on Neutrophil Extracellular Traps in Experimental Colitis" International Journal of Molecular Sciences 22, no. 23: 12729. https://doi.org/10.3390/ijms222312729

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