Comprehensive Comparative Analysis of the GATA Transcription Factors in Four Rosaceae Species and Phytohormonal Response in Chinese Pear (Pyrus bretschneideri) Fruit
Abstract
:1. Introduction
2. Results
2.1. Identification and Classification of GATA Gene Family in Four Rosaceae Species
2.2. Gene Structure and Conserved Motifs of GATA Gene Family
2.3. GO Annotation Analysis
2.4. Chromosomal Distribution and Gene Duplication Events and Ka/Ks Analysis
2.5. Collinearity Relationships
2.6. Activity Analysis of PbGATA Promoter
2.7. Transcriptomic Pattern of the PbGATA Genes
2.8. Subcellular Localization Analysis
2.9. Gene Expression Pattern by qRT-PCR of PbGATA after Hormonal Treatment
3. Discussion
4. Materials and Methods
4.1. Database Search and Physicochemical Characterization of GATA Genes
4.2. Chromosomal Localization and Introns/Exons Analysis
4.3. Collinearity Relationships, Mode of Gene Duplications, and Synonymous (ks) and Non-Synonymous (ka) Substitution
4.4. Conserved Motifs and Promoter Sequence Analysis
4.5. In Silico Expression Analysis of Annotated PbGATA Genes
4.6. Comparative Phylogenetic Analysis
4.7. Plant Materials and Stress Treatment
4.8. Subcellular Localization of PbGATA Protein
4.9. Isolation of Total RNA and Quantitative Real-Time PCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Manzoor, M.A.; Sabir, I.A.; Shah, I.H.; Wang, H.; Yu, Z.; Rasool, F.; Mazhar, M.Z.; Younas, S.; Abdullah, M.; Cai, Y. Comprehensive Comparative Analysis of the GATA Transcription Factors in Four Rosaceae Species and Phytohormonal Response in Chinese Pear (Pyrus bretschneideri) Fruit. Int. J. Mol. Sci. 2021, 22, 12492. https://doi.org/10.3390/ijms222212492
Manzoor MA, Sabir IA, Shah IH, Wang H, Yu Z, Rasool F, Mazhar MZ, Younas S, Abdullah M, Cai Y. Comprehensive Comparative Analysis of the GATA Transcription Factors in Four Rosaceae Species and Phytohormonal Response in Chinese Pear (Pyrus bretschneideri) Fruit. International Journal of Molecular Sciences. 2021; 22(22):12492. https://doi.org/10.3390/ijms222212492
Chicago/Turabian StyleManzoor, Muhammad Aamir, Irfan Ali Sabir, Iftikhar Hussain Shah, Han Wang, Zhao Yu, Faiz Rasool, Muhammad Zaid Mazhar, Shoaib Younas, Muhammad Abdullah, and Yongping Cai. 2021. "Comprehensive Comparative Analysis of the GATA Transcription Factors in Four Rosaceae Species and Phytohormonal Response in Chinese Pear (Pyrus bretschneideri) Fruit" International Journal of Molecular Sciences 22, no. 22: 12492. https://doi.org/10.3390/ijms222212492