Expression of Specific Alleles of Zinc-Finger Transcription Factors, HvSAP8 and HvSAP16, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations
Abstract
:1. Introduction
2. Results
2.1. Bioinformatics Analysis and Molecular Phylogeny of HvSAP8 and HvSAP16 Genes
2.2. SNP Identification and Assessment in Parents of Barley Populations
2.3. SNP Genotyping of Two Barley Hybrid Populations G×B and N×A Using ASQ and Amplifluor Methods
2.4. HvSAP8 and HvSAP16 Expression in Drought-Stressed Barley Parents and Breeding Lines Selected from Hybrid Populations
2.5. Barley Breeding Lines Selected for HvSAP8 and HvSAP16 Genotypes: Drought Tolerance and Grain Yield in Dry Environment
3. Discussion
4. Materials and Methods
4.1. Bioinformatic Analysis
4.2. Plant Material
4.3. Drought Stress Treatment
4.4. DNA Extraction, Sequencing and SNP Identification
4.5. Allele-Specific qPCR (ASQ) and Amplifluor-Like SNP Genotyping
4.6. RNA Extraction, cDNA Synthesis, and qPCR Analysis
4.7. Agronomy Traits: Grain Yield and Their Components
4.8. Statistical Treatment
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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SNP Marker and Hybrid | Homozygote aa | Heterozygote ab | Homozygote bb | Total | χ2 (1:2:1, df = 2) |
---|---|---|---|---|---|
HvSAP8, G×B | |||||
Observed | 10 | 23 | 9 | 42 | |
Expected | 10.5 | 21 | 10.5 | 42 | 0.43 |
HvSAP16, N×A | |||||
Observed | 14 | 24 | 12 | 50 | |
Expected | 12.5 | 25 | 12.5 | 50 | 0.24 |
Name | Origin | Accession and Genebank 1 | Role in the Study | Brief Description | Reference |
---|---|---|---|---|---|
Granal | Kazakhstan | EBDB ID: 36487, BCC1469 | ♀, population G×B | Elite grain quality and tolerance to drought | [33] |
Baisheshek | Kazakhstan | EBDB ID: 20443, IR3303 | ♂, population G×B | Tolerance to salinity | [9] |
Natali | Russia | K-30957 | ♀, population N×A | High-yielding, large grains, early ripening | [34] |
Auksiniai-2 | Lithuania | EBDB ID: 36534, JIC4863 | ♂, population N×A | Used for mutant production | [35] |
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Baidyussen, A.; Jatayev, S.; Khassanova, G.; Amantayev, B.; Sereda, G.; Sereda, S.; Gupta, N.K.; Gupta, S.; Schramm, C.; Anderson, P.; et al. Expression of Specific Alleles of Zinc-Finger Transcription Factors, HvSAP8 and HvSAP16, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations. Int. J. Mol. Sci. 2021, 22, 12156. https://doi.org/10.3390/ijms222212156
Baidyussen A, Jatayev S, Khassanova G, Amantayev B, Sereda G, Sereda S, Gupta NK, Gupta S, Schramm C, Anderson P, et al. Expression of Specific Alleles of Zinc-Finger Transcription Factors, HvSAP8 and HvSAP16, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations. International Journal of Molecular Sciences. 2021; 22(22):12156. https://doi.org/10.3390/ijms222212156
Chicago/Turabian StyleBaidyussen, Akmaral, Satyvaldy Jatayev, Gulmira Khassanova, Bekzak Amantayev, Grigory Sereda, Sergey Sereda, Narendra K. Gupta, Sunita Gupta, Carly Schramm, Peter Anderson, and et al. 2021. "Expression of Specific Alleles of Zinc-Finger Transcription Factors, HvSAP8 and HvSAP16, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations" International Journal of Molecular Sciences 22, no. 22: 12156. https://doi.org/10.3390/ijms222212156