Next Article in Journal
Structure of Lipopolysaccharide from Liberibacter crescens Is Low Molecular Weight and Offers Insight into Candidatus Liberibacter Biology
Next Article in Special Issue
Structural Modelling of KCNQ1 and KCNH2 Double Mutant Proteins, Identified in Two Severe Long QT Syndrome Cases, Reveals New Insights into Cardiac Channelopathies
Previous Article in Journal
Molecular Pathways in Prolactinomas: Translational and Therapeutic Implications
Previous Article in Special Issue
Calcium Signaling Silencing in Atrial Fibrillation: Implications for Atrial Sodium Homeostasis
 
 
Article
Peer-Review Record

Sarcomeres Morphology and Z-Line Arrangement Disarray Induced by Ventricular Premature Contractions through the Rac2/Cofilin Pathway

Int. J. Mol. Sci. 2021, 22(20), 11244; https://doi.org/10.3390/ijms222011244
by Yu-Sheng Lin 1,2, Tzu-Hao Chang 3, Wan-Chun Ho 1, Shun-Fu Chang 4, Yung-Lung Chen 5, Shih-Tai Chang 1, Huang-Chung Chen 5, Kuo-Li Pan 1 and Mien-Cheng Chen 5,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2021, 22(20), 11244; https://doi.org/10.3390/ijms222011244
Submission received: 12 September 2021 / Revised: 11 October 2021 / Accepted: 12 October 2021 / Published: 18 October 2021

Round 1

Reviewer 1 Report

This is a very interesting paper looking at the role of disarrayed sarcomere morphology and Rac2/cofilin pathway in a pig model of VPCs.

Apart from some minor inaccuracies in the writing and punctuation, outlined below, the study represents a carefully planned and performed research worthy of publication.

 

  • references in square brackets shall be moved before the pagsemicolon/point
  • 3, Figure 2B, to change LVFM with LVFW
  • 4, line 111, to change LVF with LVFM
  • 4 (lines 136,138,139) and pag. 11 (line 37), typeface needs to be aligned to test for underlined words
  • in legends of Figures 4, 5 and 7 the P values are missing
  • 11, test from line 37 to 49 needs to be formatted
  • 13, line 105, “alteration” instead of “ alternation”
  • 13, line 115, reference [8] does not seem to represent the latter information about the heart
  • 13, line 121, “by the increased” instead of “by increased the”
  • 5 paragraph typeface needs to be aligned to test
  • In legends of Figures with western it needs to add “PC:positive control”

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

This is an important experimental study in animal models to determine the underlying pathology of PVCs.

I congratulate authors to conduct this experiment. There are few reservations on this study.

  1. Study conclusion can not be generalized as it is limited to 6 animals.
  2. The stimulus used is electrical current for 6 months which produced significant changes in the myocardium, which is not the case in isolated PVC's.
  3. The authors have studied the effect on actin myofilaments along the Z line, but not studied the beta receptors, whether there is any upregulation or down regulation of beta receptors
  4. The findings of RAc2 played a crucial role has not been confirmed in knock out model, which limits the study outcomes.
  5. Although Rac2?cofilin was found in this study to be important, other pathways or mechanisms are not studied.

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Back to TopTop