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A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells
 
 
Article

Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold

1
Department of Periodontology, School of Dental Medicine, University of Bern, 3010 Bern, Switzerland
2
Robert K. Schenk Laboratory of Oral Histology, School of Dental Medicine, University of Bern, 3010 Bern, Switzerland
3
Department of Cranio-Maxillofacial Surgery, Inselspital, Bern University Hospital, Faculty of Medicine, University of Bern, 3010 Bern, Switzerland
4
Department of Periodontology and Operative Dentistry, University Medical Centre of the Johannes Gutenberg University, 55131 Mainz, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Thimios A. Mitsiadis
Int. J. Mol. Sci. 2021, 22(20), 10915; https://doi.org/10.3390/ijms222010915
Received: 22 September 2021 / Revised: 3 October 2021 / Accepted: 7 October 2021 / Published: 9 October 2021
(This article belongs to the Special Issue Advances in Translational Research in Oral Tissue Regeneration)
(1) Aim: To immunohistochemically evaluate the effect of a volume-stable collagen scaffold (VCMX) on periodontal regeneration. (2) Methods: In eight beagle dogs, acute two-wall intrabony defects were treated with open flap debridement either with VCMX (test) or without (control). After 12 weeks, eight defects out of four animals were processed for paraffin histology and immunohistochemistry. (3) Results: All defects (four test + four control) revealed periodontal regeneration with cementum and bone formation. VCMX remnants were integrated in bone, periodontal ligament (PDL), and cementum. No differences in immunohistochemical labeling patterns were observed between test and control sites. New bone and cementum were labeled for bone sialoprotein, while the regenerated PDL was labeled for periostin and collagen type 1. Cytokeratin-positive epithelial cell rests of Malassez were detected in 50% of the defects. The regenerated PDL demonstrated a larger blood vessel area at the test (14.48% ± 3.52%) than at control sites (8.04% ± 1.85%, p = 0.0007). The number of blood vessels was higher in the regenerated PDL (test + control) compared to the pristine one (p = 0.012). The cell proliferative index was not statistically significantly different in pristine and regenerated PDL. (4) Conclusions: The data suggest a positive effect of VCMX on angiogenesis and an equally high cell turnover in the regenerated and pristine PDL. This VCMX supported periodontal regeneration in intrabony defects. View Full-Text
Keywords: periodontal regeneration; intrabony defect; collagen scaffold; volume-stable collagen matrix; biomaterial; histology; immunohistochemistry periodontal regeneration; intrabony defect; collagen scaffold; volume-stable collagen matrix; biomaterial; histology; immunohistochemistry
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MDPI and ACS Style

Imber, J.-C.; Roccuzzo, A.; Stähli, A.; Saulacic, N.; Deschner, J.; Sculean, A.; Bosshardt, D.D. Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold. Int. J. Mol. Sci. 2021, 22, 10915. https://doi.org/10.3390/ijms222010915

AMA Style

Imber J-C, Roccuzzo A, Stähli A, Saulacic N, Deschner J, Sculean A, Bosshardt DD. Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold. International Journal of Molecular Sciences. 2021; 22(20):10915. https://doi.org/10.3390/ijms222010915

Chicago/Turabian Style

Imber, Jean-Claude, Andrea Roccuzzo, Alexandra Stähli, Nikola Saulacic, James Deschner, Anton Sculean, and Dieter Daniel Bosshardt. 2021. "Immunohistochemical Evaluation of Periodontal Regeneration Using a Porous Collagen Scaffold" International Journal of Molecular Sciences 22, no. 20: 10915. https://doi.org/10.3390/ijms222010915

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