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Article

Annexin 1 Is a Component of eATP-Induced Cytosolic Calcium Elevation in Arabidopsis thaliana Roots

1
Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, UK
2
School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, China
3
RIKEN Centre for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan
4
School of Agriculture, Ludong University, Yantai 264205, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(2), 494; https://doi.org/10.3390/ijms22020494
Received: 14 December 2020 / Revised: 3 January 2021 / Accepted: 4 January 2021 / Published: 6 January 2021
(This article belongs to the Special Issue Cell Signaling in Model Plants 2.0)
Extracellular ATP (eATP) has long been established in animals as an important signalling molecule but this is less understood in plants. The identification of Arabidopsis thaliana DORN1 (Does Not Respond to Nucleotides) as the first plant eATP receptor has shown that it is fundamental to the elevation of cytosolic free Ca2+ ([Ca2+]cyt) as a possible second messenger. eATP causes other downstream responses such as increase in reactive oxygen species (ROS) and nitric oxide, plus changes in gene expression. The plasma membrane Ca2+ influx channels involved in eATP-induced [Ca2+]cyt increase remain unknown at the genetic level. Arabidopsis thaliana Annexin 1 has been found to mediate ROS-activated Ca2+ influx in root epidermis, consistent with its operating as a transport pathway. In this study, the loss of function Annexin 1 mutant was found to have impaired [Ca2+]cyt elevation in roots in response to eATP or eADP. Additionally, this annexin was implicated in modulating eATP-induced intracellular ROS accumulation in roots as well as expression of eATP-responsive genes. View Full-Text
Keywords: extracellular ATP; ADP; root; Arabidopsis; annexin 1; calcium; calcium channel; reactive oxygen species extracellular ATP; ADP; root; Arabidopsis; annexin 1; calcium; calcium channel; reactive oxygen species
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MDPI and ACS Style

Mohammad-Sidik, A.; Sun, J.; Shin, R.; Song, Z.; Ning, Y.; Matthus, E.; Wilkins, K.A.; Davies, J.M. Annexin 1 Is a Component of eATP-Induced Cytosolic Calcium Elevation in Arabidopsis thaliana Roots. Int. J. Mol. Sci. 2021, 22, 494. https://doi.org/10.3390/ijms22020494

AMA Style

Mohammad-Sidik A, Sun J, Shin R, Song Z, Ning Y, Matthus E, Wilkins KA, Davies JM. Annexin 1 Is a Component of eATP-Induced Cytosolic Calcium Elevation in Arabidopsis thaliana Roots. International Journal of Molecular Sciences. 2021; 22(2):494. https://doi.org/10.3390/ijms22020494

Chicago/Turabian Style

Mohammad-Sidik, Amirah, Jian Sun, Ryoung Shin, Zhizhong Song, Youzheng Ning, Elsa Matthus, Katie A. Wilkins, and Julia M. Davies. 2021. "Annexin 1 Is a Component of eATP-Induced Cytosolic Calcium Elevation in Arabidopsis thaliana Roots" International Journal of Molecular Sciences 22, no. 2: 494. https://doi.org/10.3390/ijms22020494

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