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Article

Characterization of the Heat-Stable Proteome during Seed Germination in Arabidopsis with Special Focus on LEA Proteins

1
Max Planck Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476 Potsdam, Germany
2
Celon Pharma S.A., Marymoncka 15, Kazun Nowy, 05-152 Czosnow, Poland
3
Institute of Life Science, University of Rostock, Albert-Einstein-Str. 3, 18059 Rostock, Germany
4
Boyce Thompson Institute, Cornell University, Ithaca, NY 14853, USA
*
Author to whom correspondence should be addressed.
We dedicate this paper to the memory of Dirk K. Hincha.
Academic Editor: Sixue Chen
Int. J. Mol. Sci. 2021, 22(15), 8172; https://doi.org/10.3390/ijms22158172
Received: 8 June 2021 / Revised: 27 July 2021 / Accepted: 28 July 2021 / Published: 29 July 2021
(This article belongs to the Special Issue Plant Proteomic Research 4.0)
During seed germination, desiccation tolerance is lost in the radicle with progressing radicle protrusion and seedling establishment. This process is accompanied by comprehensive changes in the metabolome and proteome. Germination of Arabidopsis seeds was investigated over 72 h with special focus on the heat-stable proteome including late embryogenesis abundant (LEA) proteins together with changes in primary metabolites. Six metabolites in dry seeds known to be important for seed longevity decreased during germination and seedling establishment, while all other metabolites increased simultaneously with activation of growth and development. Thermo-stable proteins were associated with a multitude of biological processes. In the heat-stable proteome, a relatively similar proportion of fully ordered and fully intrinsically disordered proteins (IDP) was discovered. Highly disordered proteins were found to be associated with functional categories development, protein, RNA and stress. As expected, the majority of LEA proteins decreased during germination and seedling establishment. However, four germination-specific dehydrins were identified, not present in dry seeds. A network analysis of proteins, metabolites and amino acids generated during the course of germination revealed a highly connected LEA protein network. View Full-Text
Keywords: late embryogenesis abundant; Arabidopsis; seed germination; metabolomics; heat-stable proteome; dehydrins; LEA transcripts; intrinsically disordered proteins late embryogenesis abundant; Arabidopsis; seed germination; metabolomics; heat-stable proteome; dehydrins; LEA transcripts; intrinsically disordered proteins
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MDPI and ACS Style

Ginsawaeng, O.; Gorka, M.; Erban, A.; Heise, C.; Brueckner, F.; Hoefgen, R.; Kopka, J.; Skirycz, A.; Hincha, D.K.; Zuther, E. Characterization of the Heat-Stable Proteome during Seed Germination in Arabidopsis with Special Focus on LEA Proteins. Int. J. Mol. Sci. 2021, 22, 8172. https://doi.org/10.3390/ijms22158172

AMA Style

Ginsawaeng O, Gorka M, Erban A, Heise C, Brueckner F, Hoefgen R, Kopka J, Skirycz A, Hincha DK, Zuther E. Characterization of the Heat-Stable Proteome during Seed Germination in Arabidopsis with Special Focus on LEA Proteins. International Journal of Molecular Sciences. 2021; 22(15):8172. https://doi.org/10.3390/ijms22158172

Chicago/Turabian Style

Ginsawaeng, Orarat, Michal Gorka, Alexander Erban, Carolin Heise, Franziska Brueckner, Rainer Hoefgen, Joachim Kopka, Aleksandra Skirycz, Dirk K. Hincha, and Ellen Zuther. 2021. "Characterization of the Heat-Stable Proteome during Seed Germination in Arabidopsis with Special Focus on LEA Proteins" International Journal of Molecular Sciences 22, no. 15: 8172. https://doi.org/10.3390/ijms22158172

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