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Article

The Rab Geranylgeranyl Transferase Beta Subunit Is Essential for Embryo and Seed Development in Arabidopsis thaliana

1
Faculty of Biology, University of Gdańsk, 80-308 Gdańsk, Poland
2
Waite Research Institute, School of Agriculture, Food and Wine, The University of Adelaide, Urrbrae, SA 5064, Australia
3
Intercollegiate Faculty of Biotechnology, University of Gdańsk, 80-307 Gdańsk, Poland
4
Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland
5
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Ana Marta Pereira
Int. J. Mol. Sci. 2021, 22(15), 7907; https://doi.org/10.3390/ijms22157907
Received: 1 July 2021 / Revised: 20 July 2021 / Accepted: 22 July 2021 / Published: 24 July 2021
(This article belongs to the Special Issue Signaling in Plant Reproduction)
Auxin is a key regulator of plant development affecting the formation and maturation of reproductive structures. The apoplastic route of auxin transport engages influx and efflux facilitators from the PIN, AUX and ABCB families. The polar localization of these proteins and constant recycling from the plasma membrane to endosomes is dependent on Rab-mediated vesicular traffic. Rab proteins are anchored to membranes via posttranslational addition of two geranylgeranyl moieties by the Rab Geranylgeranyl Transferase enzyme (RGT), which consists of RGTA, RGTB and REP subunits. Here, we present data showing that seed development in the rgtb1 mutant, with decreased vesicular transport capacity, is disturbed. Both pre- and post-fertilization events are affected, leading to a decrease in seed yield. Pollen tube recognition at the stigma and its guidance to the micropyle is compromised and the seed coat forms incorrectly. Excess auxin in the sporophytic tissues of the ovule in the rgtb1 plants leads to an increased tendency of autonomous endosperm formation in unfertilized ovules and influences embryo development in a maternal sporophytic manner. The results show the importance of vesicular traffic for sexual reproduction in flowering plants, and highlight RGTB1 as a key component of sporophytic-filial signaling. View Full-Text
Keywords: Arabidopsis; auxin; embryo; endosperm; PIN1; Rab; Rab Geranylgeranyl Transferase; seed; seed coat Arabidopsis; auxin; embryo; endosperm; PIN1; Rab; Rab Geranylgeranyl Transferase; seed; seed coat
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MDPI and ACS Style

Rojek, J.; Tucker, M.R.; Rychłowski, M.; Nowakowska, J.; Gutkowska, M. The Rab Geranylgeranyl Transferase Beta Subunit Is Essential for Embryo and Seed Development in Arabidopsis thaliana. Int. J. Mol. Sci. 2021, 22, 7907. https://doi.org/10.3390/ijms22157907

AMA Style

Rojek J, Tucker MR, Rychłowski M, Nowakowska J, Gutkowska M. The Rab Geranylgeranyl Transferase Beta Subunit Is Essential for Embryo and Seed Development in Arabidopsis thaliana. International Journal of Molecular Sciences. 2021; 22(15):7907. https://doi.org/10.3390/ijms22157907

Chicago/Turabian Style

Rojek, Joanna, Matthew R. Tucker, Michał Rychłowski, Julita Nowakowska, and Małgorzata Gutkowska. 2021. "The Rab Geranylgeranyl Transferase Beta Subunit Is Essential for Embryo and Seed Development in Arabidopsis thaliana" International Journal of Molecular Sciences 22, no. 15: 7907. https://doi.org/10.3390/ijms22157907

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