Using Zebrafish as a Disease Model to Study Fibrotic Disease
Abstract
:1. Introduction
2. Fibrotic Diseases and Underlying Mechanisms
2.1. Fibrotic Diseases
2.2. Cellular Mechanism of Fibrosis
2.3. Molecular Mechanism of Fibrosis
3. Zebrafish Models of Fibrotic Disease
3.1. Zebrafish Models of Cardiac Fibrosis
3.1.1. Cardiac Fibrosis Models by Ventricular Apex Resection (VAR)
3.1.2. Cardiac Fibrosis Models by Cryoinjury
3.2. Zebrafish Models of Liver Fibrosis
3.2.1. Alcoholic Liver Disease (ALD)
3.2.2. Non-Alcoholic Fatty Liver Disease (NAFLD)
3.2.3. Chemically Induced Liver Fibrosis Models
3.2.4. Genetic Liver Fibrosis Models
3.3. Zebrafish Models of Pancreas Fibrosis
3.4. Other Zebrafish Models of Fibrosis
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Organ-Specific Fibrotic Diseases |
Cardiac Fibrosis |
|
Kidney Fibrosis |
|
Pulmonary Fibrosis |
|
Liver and Portal Vein Fibrosis |
|
|
Larvae | Adults | |||||
---|---|---|---|---|---|---|
Organs | Induction | Detection | References | Induction | Detection | References |
Heart | Not known | Not known | Not known | Ventricular apex resection (20%) | AFOG staining | Poss 2002 [63] |
Cryoinjury | Aniline Blue or MT staining | Chablais et al., 2011 [21] | ||||
Multiple cryoinjuries | AFOG staining | Bise et al., 2020 [65] | ||||
Liver | TAA induction | Sirius Red staining & qPCR | van der Helm et al., 2018 [23] | EtOH induction | Picrosirius Red staining | Park and Kim 2019 [66] |
EtOH & NTR/MTZ ablation | IHC staining | Huang et al., 2016 [67] | EtOH & NTR/MTZ ablation | IHC staining | Huang et al., 2016 [67] | |
EtOH & mpi knockdown | qPCR | DeRossi et al., 2019 [68] | EtOH & mpi knockdown | MT staining | DeRossi et al., 2019 [68] | |
Ovarian senescence & obesity | Sirius Red staining | Turola et al., 2015 [69] | ||||
Overexpressed tgfbβ1α induction | Sirius Red & IHC staining | Yan et al., 2019 [13] | ||||
Pancreas | Not known | Not known | Not known | Hedgehog (Hh)-induction | MT staining | Jung et al., 2011 [22] |
Transgene KRASG12D expression | MT staining | Oh and Park 2019 [14] | ||||
Other organs (tissues) | Not known | Not known | Not known | PHMG-P induced gill fibrosis | MT staining | Oh et al., 2018 [70] |
Ionizing irradiation caused muscle fibrosis | MT staining | Epperly et al., 2012 [71] |
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Wang, X.; Copmans, D.; de Witte, P.A.M. Using Zebrafish as a Disease Model to Study Fibrotic Disease. Int. J. Mol. Sci. 2021, 22, 6404. https://doi.org/10.3390/ijms22126404
Wang X, Copmans D, de Witte PAM. Using Zebrafish as a Disease Model to Study Fibrotic Disease. International Journal of Molecular Sciences. 2021; 22(12):6404. https://doi.org/10.3390/ijms22126404
Chicago/Turabian StyleWang, Xixin, Daniëlle Copmans, and Peter A. M. de Witte. 2021. "Using Zebrafish as a Disease Model to Study Fibrotic Disease" International Journal of Molecular Sciences 22, no. 12: 6404. https://doi.org/10.3390/ijms22126404