The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of Ustilaginoidea virens Regulates Defense- and Heading Date-Related Signaling Pathway

Ustilaginoidea virens, which causes rice false smut (RFS), is one of the most detrimental rice fungal diseases and poses a severe threat to rice production and quality. Effectors in U. virens often act as a group of essential virulence factors that play crucial roles in the interaction between host and the pathogen. Thus, the functions of individual effectors in U. virens need to be further explored. Here, we demonstrated a small secreted hypersensitive response-inducing protein (hrip), named UvHrip1, which was highly conserved in U. virens isolates. UvHrip1 was also proven to suppress necrosis-like defense symptoms in N. benthamiana induced by the oomycete elicitor INF1. The localization of UvHrip1 was mainly in the nuclei and cytoplasm via monitoring the UvHrip1-GFP fusion protein in rice cells. Furthermore, Y2H and BiFC assay demonstrated that UvHrip1 interacted with OsHGW, which is a critical regulator in heading date and grain weight signaling pathways in rice. Expression patterns of defense- and heading date-related genes, OsPR1#051 and OsMYB21, were down-regulated over U. virens infection in rice. Collectively, our data provide a theory for gaining an insight into the molecular mechanisms underlying the UvHrip1 virulence function. View Full-Text

Keywords: effector; Ustilaginoidea virens; flowering time; innate immunity; pathogenicity

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Wei, S.; Wang, Y.; Zhou, J.; Xiang, S.; Sun, W.; Peng, X.; Li, J.; Hai, Y.; Wang, Y.; Li, S. The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of Ustilaginoidea virens Regulates Defense- and Heading Date-Related Signaling Pathway. Int. J. Mol. Sci. 2020, 21, 3376. https://doi.org/10.3390/ijms21093376

AMA Style

Wei S, Wang Y, Zhou J, Xiang S, Sun W, Peng X, Li J, Hai Y, Wang Y, Li S. The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of Ustilaginoidea virens Regulates Defense- and Heading Date-Related Signaling Pathway. International Journal of Molecular Sciences. 2020; 21(9):3376. https://doi.org/10.3390/ijms21093376

Chicago/Turabian Style

Wei, Songhong, Yingling Wang, Jianming Zhou, Shibo Xiang, Wenxian Sun, Xunwen Peng, Jing Li, Yingfan Hai, Yan Wang, and Shuai Li. 2020. "The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of Ustilaginoidea virens Regulates Defense- and Heading Date-Related Signaling Pathway" International Journal of Molecular Sciences 21, no. 9: 3376. https://doi.org/10.3390/ijms21093376

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The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of Ustilaginoidea virens Regulates Defense- and Heading Date-Related Signaling Pathway

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