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Article

Kandelia candel Thioredoxin f Confers Osmotic Stress Tolerance in Transgenic Tobacco

1
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
2
Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Qingdao 266237, China
3
Urat Desert-Grassland Research Station, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Science, Lanzhou 730000, China
4
Department of Biology, College of Life Science, Hainan Normal University, Haikou 571158, China
5
Beijing Research Center of Intelligent Equipment for Agriculture, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(9), 3335; https://doi.org/10.3390/ijms21093335
Received: 11 April 2020 / Revised: 30 April 2020 / Accepted: 6 May 2020 / Published: 8 May 2020
(This article belongs to the Special Issue Antioxidant Metabolic Pathways in Plants)
Water deficit caused by osmotic stress and drought limits crop yield and tree growth worldwide. Screening and identifying candidate genes from stress-resistant species are a genetic engineering strategy to increase drought resistance. In this study, an increased concentration of mannitol resulted in elevated expression of thioredoxin f (KcTrxf) in the nonsecretor mangrove species Kandelia candel. By means of amino acid sequence and phylogenetic analysis, the mangrove Trx was classified as an f-type thioredoxin. Subcellular localization showed that KcTrxf localizes to chloroplasts. Enzymatic activity characterization revealed that KcTrxf recombinant protein possesses the disulfide reductase function. KcTrxf overexpression contributes to osmotic and drought tolerance in tobacco in terms of fresh weight, root length, malondialdehyde (MDA) content, and hydrogen peroxide (H2O2) production. KcTrxf was shown to reduce the stomatal aperture by enhancing K+ efflux in guard cells, which increased the water-retaining capacity in leaves under drought conditions. Notably, the abscisic acid (ABA) sensitivity was increased in KcTrxf-transgenic tobacco, which benefits plants exposed to drought by reducing water loss by promoting stomatal closure. KcTrxf-transgenic plants limited drought-induced H2O2 in leaves, which could reduce lipid peroxidation and retain the membrane integrity. Additionally, glutathione (GSH) contributing to reactive oxygen species (ROS) scavenging and transgenic plants are more efficient at regenerating GSH from oxidized glutathione (GSSG) under conditions of drought stress. Notably, KcTrxf-transgenic plants had increased glucose and fructose contents under drought stress conditions, presumably resulting from KcTrxf-promoted starch degradation under water stress. We conclude that KcTrxf contributes to drought tolerance by increasing the water status, by enhancing osmotic adjustment, and by maintaining ROS homeostasis in transgene plants. View Full-Text
Keywords: thioredoxin; Kandelia candel; mannitol; drought; water retaining capacity; soluble sugar; H2O2; stomatal aperture; abscisic acid; K+ flux; guard cells; noninvasive micro-test technique thioredoxin; Kandelia candel; mannitol; drought; water retaining capacity; soluble sugar; H2O2; stomatal aperture; abscisic acid; K+ flux; guard cells; noninvasive micro-test technique
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MDPI and ACS Style

Jing, X.; Yao, J.; Ma, X.; Zhang, Y.; Sun, Y.; Xiang, M.; Hou, P.; Li, N.; Zhao, R.; Li, J.; Zhou, X.; Chen, S. Kandelia candel Thioredoxin f Confers Osmotic Stress Tolerance in Transgenic Tobacco. Int. J. Mol. Sci. 2020, 21, 3335. https://doi.org/10.3390/ijms21093335

AMA Style

Jing X, Yao J, Ma X, Zhang Y, Sun Y, Xiang M, Hou P, Li N, Zhao R, Li J, Zhou X, Chen S. Kandelia candel Thioredoxin f Confers Osmotic Stress Tolerance in Transgenic Tobacco. International Journal of Molecular Sciences. 2020; 21(9):3335. https://doi.org/10.3390/ijms21093335

Chicago/Turabian Style

Jing, Xiaoshu, Jun Yao, Xujun Ma, Yanli Zhang, Yuanling Sun, Min Xiang, Peichen Hou, Niya Li, Rui Zhao, Jinke Li, Xiaoyang Zhou, and Shaoliang Chen. 2020. "Kandelia candel Thioredoxin f Confers Osmotic Stress Tolerance in Transgenic Tobacco" International Journal of Molecular Sciences 21, no. 9: 3335. https://doi.org/10.3390/ijms21093335

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