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Article

Polysaccharide-Based Injection Matrix for Serial Crystallography

Department of Life Science, Pohang University of Science and Technology, Pohang 37673, Korea
Int. J. Mol. Sci. 2020, 21(9), 3332; https://doi.org/10.3390/ijms21093332
Received: 11 April 2020 / Revised: 2 May 2020 / Accepted: 5 May 2020 / Published: 8 May 2020
(This article belongs to the Section Materials Science)
Serial crystallography (SX) provides an opportunity to observe the molecular dynamics of macromolecular structures at room temperature via pump-probe studies. The delivery of crystals embedded in a viscous medium via an injector or syringe is widely performed in synchrotrons or X-ray free-electron laser facilities with low repetition rates. Various viscous media have been developed; however, there are cases in which the delivery material undesirably interacts chemically or biologically with specific protein samples, or changes the stability of the injection stream, depending on the crystallization solution. Therefore, continued discovery and characterization of new delivery media is necessary for expanding future SX applications. Here, the preparation and characterization of new polysaccharide (wheat starch (WS) and alginate)-based sample delivery media are introduced for SX. Crystals embedded in a WS or alginate injection medium showed a stable injection stream at a flow rate of < 200 nL/min and low-level X-ray background scattering similar to other hydrogels. Using these media, serial millisecond crystallography (SMX) was performed, and the room temperature crystal structures of glucose isomerase and lysozyme were determined at 1.9–2.0 Å resolutions. WS and alginate will allow an expanded application of sample delivery media in SX experiments. View Full-Text
Keywords: serial crystallography; sample delivery; viscous medium; wheat starch; alginate; SMX; SFX; background scattering serial crystallography; sample delivery; viscous medium; wheat starch; alginate; SMX; SFX; background scattering
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MDPI and ACS Style

Nam, K.H. Polysaccharide-Based Injection Matrix for Serial Crystallography. Int. J. Mol. Sci. 2020, 21, 3332. https://doi.org/10.3390/ijms21093332

AMA Style

Nam KH. Polysaccharide-Based Injection Matrix for Serial Crystallography. International Journal of Molecular Sciences. 2020; 21(9):3332. https://doi.org/10.3390/ijms21093332

Chicago/Turabian Style

Nam, Ki H. 2020. "Polysaccharide-Based Injection Matrix for Serial Crystallography" International Journal of Molecular Sciences 21, no. 9: 3332. https://doi.org/10.3390/ijms21093332

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