Next Article in Journal
Genome-Wide Identification of M35 Family Metalloproteases in Rhizoctonia cerealis and Functional Analysis of RcMEP2 as a Virulence Factor during the Fungal Infection to Wheat
Next Article in Special Issue
The EDCMET Project: Metabolic Effects of Endocrine Disruptors
Previous Article in Journal
SGLT2 Inhibitor Empagliflozin and DPP4 Inhibitor Linagliptin Reactivate Glomerular Autophagy in db/db Mice, a Model of Type 2 Diabetes
Previous Article in Special Issue
ERGO: Breaking Down the Wall between Human Health and Environmental Testing of Endocrine Disrupters
Project Report

Integrative Strategy of Testing Systems for Identification of Endocrine Disruptors Inducing Metabolic Disorders—An Introduction to the OBERON Project

1
Inserm UMR S-1124, Université de Paris, 75006 Paris, France
2
HERACLES Research Center on the Exposome and Health, Aristotle University of Thessaloniki, Center for Interdisciplinary Research and Innovation, 57001 Thessaloniki, Greece
3
Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE), Universidad Miguel Hernández, 03202 Elche, Spain
4
Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), 28029 Madrid, Spain
5
Institut National de l’Environnement Industriel et des Risques (INERIS), Unité Modèles pour l’Ecotoxicologie et la Toxicologie (METO), Parc ALATA BP2, 60550 Verneuil en Halatte, France
6
ISGlobal, 08003 Barcelona, Spain
7
Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain
8
CIBER Epidemiología y Salud Pública (CIBERESP), 28029 Madrid, Spain
9
Department of Life and Health Sciences, University of Bordeaux, INSERM U1211, MRGM, F-33615 Pessac, France
10
Enve.X, 55133 Thessaloniki, Greece
11
Service de Biochimie métabolomique et protéomique, Hôpital Necker enfants malades, AP-HP, 75015 Paris, France
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(8), 2988; https://doi.org/10.3390/ijms21082988
Received: 26 March 2020 / Revised: 19 April 2020 / Accepted: 21 April 2020 / Published: 23 April 2020
(This article belongs to the Special Issue Advances in the Research of Endocrine Disrupting Chemicals 2.0)
Exposure to chemical substances that can produce endocrine disrupting effects represents one of the most critical public health threats nowadays. In line with the regulatory framework implemented within the European Union (EU) to reduce the levels of endocrine disruptors (EDs) for consumers, new and effective methods for ED testing are needed. The OBERON project will build an integrated testing strategy (ITS) to detect ED-related metabolic disorders by developing, improving and validating a battery of test systems. It will be based on the concept of an integrated approach for testing and assessment (IATA). OBERON will combine (1) experimental methods (in vitro, e.g., using 2D and 3D human-derived cells and tissues, and in vivo, i.e., using zebrafish at different stages), (2) high throughput omics technologies, (3) epidemiology and human biomonitoring studies and (4) advanced computational models (in silico and systems biology) on functional endpoints related to metabolism. Such interdisciplinary framework will help in deciphering EDs based on a mechanistic understanding of toxicity by providing and making available more effective alternative test methods relevant for human health that are in line with regulatory needs. Data generated in OBERON will also allow the development of novel adverse outcome pathways (AOPs). The assays will be pre-validated in order to select the test systems that will show acceptable performance in terms of relevance for the second step of the validation process, i.e., the inter-laboratory validation as ring tests. Therefore, the aim of the OBERON project is to support the organization for economic co-operation and development (OECD) conceptual framework for testing and assessment of single and/or mixture of EDs by developing specific assays not covered by the current tests, and to propose an IATA for ED-related metabolic disorder detection, which will be submitted to the Joint Research Center (JRC) and OECD community. View Full-Text
Keywords: endocrine disruptors; metabolism; integrative approach; predictive toxicology; metabolic disorders; obesity; non-alcoholic fatty liver diseases; non-animal testing; adverse outcome pathways; computational systems toxicology endocrine disruptors; metabolism; integrative approach; predictive toxicology; metabolic disorders; obesity; non-alcoholic fatty liver diseases; non-animal testing; adverse outcome pathways; computational systems toxicology
Show Figures

Figure 1

MDPI and ACS Style

Audouze, K.; Sarigiannis, D.; Alonso-Magdalena, P.; Brochot, C.; Casas, M.; Vrijheid, M.; Babin, P.J.; Karakitsios, S.; Coumoul, X.; Barouki, R. Integrative Strategy of Testing Systems for Identification of Endocrine Disruptors Inducing Metabolic Disorders—An Introduction to the OBERON Project. Int. J. Mol. Sci. 2020, 21, 2988. https://doi.org/10.3390/ijms21082988

AMA Style

Audouze K, Sarigiannis D, Alonso-Magdalena P, Brochot C, Casas M, Vrijheid M, Babin PJ, Karakitsios S, Coumoul X, Barouki R. Integrative Strategy of Testing Systems for Identification of Endocrine Disruptors Inducing Metabolic Disorders—An Introduction to the OBERON Project. International Journal of Molecular Sciences. 2020; 21(8):2988. https://doi.org/10.3390/ijms21082988

Chicago/Turabian Style

Audouze, Karine; Sarigiannis, Denis; Alonso-Magdalena, Paloma; Brochot, Celine; Casas, Maribel; Vrijheid, Martine; Babin, Patrick J.; Karakitsios, Spyros; Coumoul, Xavier; Barouki, Robert. 2020. "Integrative Strategy of Testing Systems for Identification of Endocrine Disruptors Inducing Metabolic Disorders—An Introduction to the OBERON Project" Int. J. Mol. Sci. 21, no. 8: 2988. https://doi.org/10.3390/ijms21082988

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop