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Recombinant Human Plasma Gelsolin Stimulates Phagocytosis while Diminishing Excessive Inflammatory Responses in Mice with Pseudomonas aeruginosa Sepsis

1
Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Bialystok, Mickiewicza 2c, 15-222 Bialystok, Poland
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Department of Microbiology and Immunology, the Faculty of Medicine and Health Sciences of the Jan Kochanowski University in Kielce, Stefana Żeromskiego 5, 25-001 Kielce, Poland
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Department of Anatomy, Medical University of Bialystok, Mickiewicza 2b, 15-222 Bialystok, Poland
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Institute of Chemistry, University of Białystok, Ciołkowskiego 1K, 15-245 Bialystok, Poland
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Institute for Medicine and Engineering, University of Pennsylvania, 3340 Smith Walk, Philadelphia, PA 19104, USA
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Department of Pathology, Medical University of Bialystok, Waszyngtona 13, 15-269 Bialystok, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(7), 2551; https://doi.org/10.3390/ijms21072551
Received: 13 February 2020 / Revised: 29 March 2020 / Accepted: 3 April 2020 / Published: 7 April 2020
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Plasma gelsolin (pGSN) is a highly conserved abundant circulating protein, characterized by diverse immunomodulatory activities including macrophage activation and the ability to neutralize pro-inflammatory molecules produced by the host and pathogen. Using a murine model of Gram-negative sepsis initiated by the peritoneal instillation of Pseudomonas aeruginosa Xen 5, we observed a decrease in the tissue uptake of IRDye®800CW 2-deoxyglucose, an indicator of inflammation, and a decrease in bacterial growth from ascitic fluid in mice treated with intravenous recombinant human plasma gelsolin (pGSN) compared to the control vehicle. Pretreatment of the murine macrophage line RAW264.7 with pGSN, followed by addition of Pseudomonas aeruginosa Xen 5, resulted in a dose-dependent increase in the proportion of macrophages with internalized bacteria. This increased uptake was less pronounced when cells were pretreated with pGSN and then centrifuged to remove unbound pGSN before addition of bacteria to macrophages. These observations suggest that recombinant plasma gelsolin can modulate the inflammatory response while at the same time augmenting host antibacterial activity. View Full-Text
Keywords: plasma gelsolin; inflammation; phagocytosis; sepsis; Pseudomonas aeruginosa plasma gelsolin; inflammation; phagocytosis; sepsis; Pseudomonas aeruginosa
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MDPI and ACS Style

Piktel, E.; Wnorowska, U.; Cieśluk, M.; Deptuła, P.; Prasad, S.V.; Król, G.; Durnaś, B.; Namiot, A.; Markiewicz, K.H.; Niemirowicz-Laskowska, K.; Wilczewska, A.Z.; Janmey, P.A.; Reszeć, J.; Bucki, R. Recombinant Human Plasma Gelsolin Stimulates Phagocytosis while Diminishing Excessive Inflammatory Responses in Mice with Pseudomonas aeruginosa Sepsis. Int. J. Mol. Sci. 2020, 21, 2551. https://doi.org/10.3390/ijms21072551

AMA Style

Piktel E, Wnorowska U, Cieśluk M, Deptuła P, Prasad SV, Król G, Durnaś B, Namiot A, Markiewicz KH, Niemirowicz-Laskowska K, Wilczewska AZ, Janmey PA, Reszeć J, Bucki R. Recombinant Human Plasma Gelsolin Stimulates Phagocytosis while Diminishing Excessive Inflammatory Responses in Mice with Pseudomonas aeruginosa Sepsis. International Journal of Molecular Sciences. 2020; 21(7):2551. https://doi.org/10.3390/ijms21072551

Chicago/Turabian Style

Piktel, Ewelina, Urszula Wnorowska, Mateusz Cieśluk, Piotr Deptuła, Suhanya V. Prasad, Grzegorz Król, Bonita Durnaś, Andrzej Namiot, Karolina H. Markiewicz, Katarzyna Niemirowicz-Laskowska, Agnieszka Z. Wilczewska, Paul A. Janmey, Joanna Reszeć, and Robert Bucki. 2020. "Recombinant Human Plasma Gelsolin Stimulates Phagocytosis while Diminishing Excessive Inflammatory Responses in Mice with Pseudomonas aeruginosa Sepsis" International Journal of Molecular Sciences 21, no. 7: 2551. https://doi.org/10.3390/ijms21072551

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