SIRT2 Affects Primary Cilia Formation by Regulating mTOR Signaling in Retinal Pigmented Epithelial Cells

Lim, Jeaho; Son, Juyoung; Ryu, Jaewook; Kim, Ja-Eun

doi:10.3390/ijms21062240

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SIRT2 Affects Primary Cilia Formation by Regulating mTOR Signaling in Retinal Pigmented Epithelial Cells

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Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Korea

Department of Pharmacology, School of Medicine, Kyung Hee University, Seoul 02447, Korea

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Int. J. Mol. Sci. 2020, 21(6), 2240; https://doi.org/10.3390/ijms21062240

Received: 8 March 2020 / Revised: 18 March 2020 / Accepted: 20 March 2020 / Published: 24 March 2020

(This article belongs to the Section Molecular Biology)

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Abstract

SIRT2, a member of the Class III HDAC family, participates in diverse cellular processes and regulates several pathological conditions. Although a few reports show that SIRT2 regulates the cell cycle, the causes and outcomes of SIRT2-dependent cell proliferation remain unclear. Here, we examined the effects of SIRT2 suppression in human RPE1 cells using siRNA targeting SIRT2, and AK-1, a SIRT2-specific inhibitor. The number of primary cilia in SIRT2-suppressed cells increased under serum-present conditions. Suppressing SIRT2 induced cell cycle arrest at G0/G1 phase by inactivating mammalian target of rapamycin (mTOR) signaling, possibly through mTORC1. Treatment with torin 1, an inhibitor of mTORC1/mTORC2, yielded results similar to those observed after SIRT2 suppression. However, SIRT2 suppression did not affect primary cilia formation or mTOR signaling following serum starvation. This suggests that SIRT2 acts as a critical sensor that links growth factor-dependent signal transduction and primary cilia formation by regulating the cell cycle. View Full-Text

Keywords: SIRT2; mTOR; cilia; cell cycle

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MDPI and ACS Style

Lim, J.; Son, J.; Ryu, J.; Kim, J.-E. SIRT2 Affects Primary Cilia Formation by Regulating mTOR Signaling in Retinal Pigmented Epithelial Cells. Int. J. Mol. Sci. 2020, 21, 2240. https://doi.org/10.3390/ijms21062240

AMA Style

Lim J, Son J, Ryu J, Kim J-E. SIRT2 Affects Primary Cilia Formation by Regulating mTOR Signaling in Retinal Pigmented Epithelial Cells. International Journal of Molecular Sciences. 2020; 21(6):2240. https://doi.org/10.3390/ijms21062240

Chicago/Turabian Style

Lim, Jeaho, Juyoung Son, Jaewook Ryu, and Ja-Eun Kim. 2020. "SIRT2 Affects Primary Cilia Formation by Regulating mTOR Signaling in Retinal Pigmented Epithelial Cells" International Journal of Molecular Sciences 21, no. 6: 2240. https://doi.org/10.3390/ijms21062240

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SIRT2 Affects Primary Cilia Formation by Regulating mTOR Signaling in Retinal Pigmented Epithelial Cells

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