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Preparation and Characterization of Aptamers Against O,p’-DDT

School of Medicine, Huaqiao University, Quanzhou 362021, Fujian, China
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(6), 2211; https://doi.org/10.3390/ijms21062211
Received: 21 January 2020 / Revised: 11 March 2020 / Accepted: 19 March 2020 / Published: 23 March 2020
(This article belongs to the Section Molecular Toxicology)
The compound 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) ethane (o,p’-DDT) has been identified as one of the endocrine-disrupting chemicals causing adverse effects on wildlife and even humans through bioaccumulation. Its detection has become increasingly important. We have obtained candidate aptamers binding to o,p’-DDT by a systematic evolution of ligands by exponential enrichment (SELEX) protocol. Five out of seventeen candidate sequences were selected for preliminary characterization by SYBR Green I assay. One sequence with highest fluorescence response with o,p’-DDT, designated DDT_13, was chosen for further characterization. Its dissociation constant (Kd) was determined to be 412.3 ± 124.6 nM. DDT_13 exhibited low cross-binding activities on other tested small molecules. The good bioactivities of DDT_13 were demonstrated for the analysis of spiked lake water and tap water samples. This study provides a novel o,p’-DDT-specific probe for its future applications. View Full-Text
Keywords: o,p’-DDT; aptamer; SELEX; AuNPs; biosensor; real water sample o,p’-DDT; aptamer; SELEX; AuNPs; biosensor; real water sample
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Zhang, W.; Li, D.; Zhang, J.; Jiang, L.; Li, Z.; Lin, J.S. Preparation and Characterization of Aptamers Against O,p’-DDT. Int. J. Mol. Sci. 2020, 21, 2211.

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