Next Article in Journal
Comparative Transcriptome Analysis Provides Insights into the Seed Germination in Cotton in Response to Chilling Stress
Next Article in Special Issue
Comparative Transcriptome Analysis Reveals the Potential Mechanism of Abortion in Tobacco sua-Cytoplasmic Male Sterility
Previous Article in Journal
Resvega Alleviates Hydroquinone-Induced Oxidative Stress in ARPE-19 Cells
Previous Article in Special Issue
Distinct Differentiation Characteristics of Endothelium Determine Its Ability to Form Pseudo-Embryos in Tomato Ovules
Open AccessArticle

The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana

1
Laboratory of Pollen Biology, Institute of Experimental Botany of the Czech Academy of Sciences, 16502 Praha 6, Czech Republic
2
Department of Experimental Plant Biology, Faculty of Science, Charles University, 12800 Praha 2, Czech Republic
3
Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, 62500 Brno, Czech Republic
4
Station of Apple Breeding for Disease Resistance, Institute of Experimental Botany of the Czech Academy of Sciences, 16502 Praha 6, Czech Republic
5
Laboratory of Functional Genomics and Proteomics, National Centre for Biomolecular Research, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(6), 2065; https://doi.org/10.3390/ijms21062065
Received: 14 February 2020 / Revised: 11 March 2020 / Accepted: 14 March 2020 / Published: 17 March 2020
(This article belongs to the Special Issue Plant Fertility and Sexual Reproduction)
The nascent polypeptide-associated (NAC) complex was described in yeast as a heterodimer composed of two subunits, α and β, and was shown to bind to the nascent polypeptides newly emerging from the ribosomes. NAC function was widely described in yeast and several information are also available about its role in plants. The knock down of individual NAC subunit(s) led usually to a higher sensitivity to stress. In Arabidopsis thaliana genome, there are five genes encoding NACα subunit, and two genes encoding NACβ. Double homozygous mutant in both genes coding for NACβ was acquired, which showed a delayed development compared to the wild type, had abnormal number of flower organs, shorter siliques and greatly reduced seed set. Both NACβ genes were characterized in more detail—the phenotype of the double homozygous mutant was complemented by a functional NACβ copy. Then, both NACβ genes were localized to nuclei and cytoplasm and their promoters were active in many organs (leaves, cauline leaves, flowers, pollen grains, and siliques together with seeds). Since flowers were the most affected organs by nacβ mutation, the flower buds’ transcriptome was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NACβ subunits in stress responses, male gametophyte development, and photosynthesis. View Full-Text
Keywords: Arabidopsis thaliana; chaperone; flower bud proteome; flower bud transcriptome; male gametophyte; nascent polypeptide-associated complex Arabidopsis thaliana; chaperone; flower bud proteome; flower bud transcriptome; male gametophyte; nascent polypeptide-associated complex
Show Figures

Figure 1

MDPI and ACS Style

Fíla, J.; Klodová, B.; Potěšil, D.; Juříček, M.; Šesták, P.; Zdráhal, Z.; Honys, D. The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana. Int. J. Mol. Sci. 2020, 21, 2065. https://doi.org/10.3390/ijms21062065

AMA Style

Fíla J, Klodová B, Potěšil D, Juříček M, Šesták P, Zdráhal Z, Honys D. The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana. International Journal of Molecular Sciences. 2020; 21(6):2065. https://doi.org/10.3390/ijms21062065

Chicago/Turabian Style

Fíla, Jan; Klodová, Božena; Potěšil, David; Juříček, Miloslav; Šesták, Petr; Zdráhal, Zbyněk; Honys, David. 2020. "The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana" Int. J. Mol. Sci. 21, no. 6: 2065. https://doi.org/10.3390/ijms21062065

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop