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Volume 21
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10.3390/ijms21062049
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Article

Cryptides Identified in Human Apolipoprotein B as New Weapons to Fight Antibiotic Resistance in Cystic Fibrosis Disease

by
Rosa Gaglione
1,3,
Angela Cesaro
1,
Eliana Dell’Olmo
1,
Rocco Di Girolamo
1,
Luca Tartaglione
1,
Elio Pizzo
2 and
Angela Arciello
1,3,*
1
Department of Chemical Sciences, University of Naples Federico II, Via Cintia 21, I-80126 Naples, Italy
2
Department of Biology, University of Naples Federico II, Via Cintia 21, I-80126 Naples, Italy
3
Istituto Nazionale di Biostrutture e Biosistemi (INBB), I-00136 Rome, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(6), 2049; https://doi.org/10.3390/ijms21062049
Submission received: 29 February 2020 / Revised: 12 March 2020 / Accepted: 13 March 2020 / Published: 17 March 2020
(This article belongs to the Special Issue Peptides for Health Benefits 2019)
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Abstract

Chronic respiratory infections are the main cause of morbidity and mortality in cystic fibrosis (CF) patients, and are characterized by the development of multidrug resistance (MDR) phenotype and biofilm formation, generally recalcitrant to treatment with conventional antibiotics. Hence, novel effective strategies are urgently needed. Antimicrobial peptides represent new promising therapeutic agents. Here, we analyze for the first time the efficacy of three versions of a cryptide identified in human apolipoprotein B (ApoB, residues 887-922) towards bacterial strains clinically isolated from CF patients. Antimicrobial and anti-biofilm properties of ApoB-derived cryptides have been analyzed by broth microdilution assays, crystal violet assays, confocal laser scanning microscopy and scanning electron microscopy. Cell proliferation assays have been performed to test cryptide effects on human host cells. ApoB-derived cryptides have been found to be endowed with significant antimicrobial and anti-biofilm properties towards Pseudomonas and Burkholderia strains clinically isolated from CF patients. Peptides have been also found to be able to act in combination with the antibiotic ciprofloxacin, and they are harmless when tested on human bronchial epithelial mesothelial cells. These findings open interesting perspectives to cryptide applicability in the treatment of chronic lung infections associated with CF disease.
Keywords: antibiotic resistance; cystic fibrosis; antimicrobial peptides; host defense peptides; cryptides; anti-biofilm peptides; synergistic effects antibiotic resistance; cystic fibrosis; antimicrobial peptides; host defense peptides; cryptides; anti-biofilm peptides; synergistic effects
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MDPI and ACS Style

Gaglione, R.; Cesaro, A.; Dell’Olmo, E.; Di Girolamo, R.; Tartaglione, L.; Pizzo, E.; Arciello, A. Cryptides Identified in Human Apolipoprotein B as New Weapons to Fight Antibiotic Resistance in Cystic Fibrosis Disease. Int. J. Mol. Sci. 2020, 21, 2049. https://doi.org/10.3390/ijms21062049

AMA Style

Gaglione R, Cesaro A, Dell’Olmo E, Di Girolamo R, Tartaglione L, Pizzo E, Arciello A. Cryptides Identified in Human Apolipoprotein B as New Weapons to Fight Antibiotic Resistance in Cystic Fibrosis Disease. International Journal of Molecular Sciences. 2020; 21(6):2049. https://doi.org/10.3390/ijms21062049

Chicago/Turabian Style

Gaglione, Rosa, Angela Cesaro, Eliana Dell’Olmo, Rocco Di Girolamo, Luca Tartaglione, Elio Pizzo, and Angela Arciello. 2020. "Cryptides Identified in Human Apolipoprotein B as New Weapons to Fight Antibiotic Resistance in Cystic Fibrosis Disease" International Journal of Molecular Sciences 21, no. 6: 2049. https://doi.org/10.3390/ijms21062049

APA Style

Gaglione, R., Cesaro, A., Dell’Olmo, E., Di Girolamo, R., Tartaglione, L., Pizzo, E., & Arciello, A. (2020). Cryptides Identified in Human Apolipoprotein B as New Weapons to Fight Antibiotic Resistance in Cystic Fibrosis Disease. International Journal of Molecular Sciences, 21(6), 2049. https://doi.org/10.3390/ijms21062049

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