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Article

Transcriptome Analysis and Identification of Genes Associated with Starch Metabolism in Castanea henryi Seed (Fagaceae)

by
Bin Liu
1,2,
Ruqiang Lin
1,2,
Yuting Jiang
1,2,
Shuzhen Jiang
1,2,
Yuanfang Xiong
1,2,
Hui Lian
1,2,
Qinmeng Zeng
1,2,
Xuedie Liu
1,2,
Zhong-Jian Liu
2 and
Shipin Chen
1,2,*
1
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape, Fujian Agriculture and Forestry University, Fuzhou 350002, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(4), 1431; https://doi.org/10.3390/ijms21041431
Submission received: 19 January 2020 / Revised: 17 February 2020 / Accepted: 18 February 2020 / Published: 20 February 2020
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Starch is the most important form of carbohydrate storage and is the major energy reserve in some seeds, especially Castanea henryi. Seed germination is the beginning of the plant’s life cycle, and starch metabolism is important for seed germination. As a complex metabolic pathway, the regulation of starch metabolism in C. henryi is still poorly understood. To explore the mechanism of starch metabolism during the germination of C. henryi, we conducted a comparative gene expression analysis at the transcriptional level using RNA-seq across four different germination stages, and analyzed the changes in the starch and soluble sugar contents. The results showed that the starch content increased in 0–10 days and decreased in 10–35 days, while the soluble sugar content continuously decreased in 0–30 days and increased in 30–35 days. We identified 49 candidate genes that may be associated with starch and sucrose metabolism. Three ADP-glucose pyrophosphorylase (AGPase) genes, two nucleotide pyrophosphatase/phosphodiesterases (NPPS) genes and three starch synthases (SS) genes may be related to starch accumulation. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the expression levels of these genes. Our study combined transcriptome data with physiological and biochemical data, revealing potential candidate genes that affect starch metabolism during seed germination, and provides important data about starch metabolism and seed germination in seed plants.
Keywords: Castanea henryi; seed germination; starch; transcriptome analysis Castanea henryi; seed germination; starch; transcriptome analysis

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MDPI and ACS Style

Liu, B.; Lin, R.; Jiang, Y.; Jiang, S.; Xiong, Y.; Lian, H.; Zeng, Q.; Liu, X.; Liu, Z.-J.; Chen, S. Transcriptome Analysis and Identification of Genes Associated with Starch Metabolism in Castanea henryi Seed (Fagaceae). Int. J. Mol. Sci. 2020, 21, 1431. https://doi.org/10.3390/ijms21041431

AMA Style

Liu B, Lin R, Jiang Y, Jiang S, Xiong Y, Lian H, Zeng Q, Liu X, Liu Z-J, Chen S. Transcriptome Analysis and Identification of Genes Associated with Starch Metabolism in Castanea henryi Seed (Fagaceae). International Journal of Molecular Sciences. 2020; 21(4):1431. https://doi.org/10.3390/ijms21041431

Chicago/Turabian Style

Liu, Bin, Ruqiang Lin, Yuting Jiang, Shuzhen Jiang, Yuanfang Xiong, Hui Lian, Qinmeng Zeng, Xuedie Liu, Zhong-Jian Liu, and Shipin Chen. 2020. "Transcriptome Analysis and Identification of Genes Associated with Starch Metabolism in Castanea henryi Seed (Fagaceae)" International Journal of Molecular Sciences 21, no. 4: 1431. https://doi.org/10.3390/ijms21041431

APA Style

Liu, B., Lin, R., Jiang, Y., Jiang, S., Xiong, Y., Lian, H., Zeng, Q., Liu, X., Liu, Z.-J., & Chen, S. (2020). Transcriptome Analysis and Identification of Genes Associated with Starch Metabolism in Castanea henryi Seed (Fagaceae). International Journal of Molecular Sciences, 21(4), 1431. https://doi.org/10.3390/ijms21041431

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