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Open AccessArticle

NCAPG Dynamically Coordinates the Myogenesis of Fetal Bovine Tissue by Adjusting Chromatin Accessibility

by Xin Hu 1,2,†, Yishen Xing 1,†, Xing Fu 3, Qiyuan Yang 4, Ling Ren 1, Yahui Wang 1, Qian Li 1, Junya Li 1,* and Lupei Zhang 1,*
1
Key Laboratory of Animal Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
Molecular and Cellular Biology, Gembloux Agro-Bio Tech, University of Liège, 5030 Gembloux, Belgium
3
School of Animal Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA
4
Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(4), 1248; https://doi.org/10.3390/ijms21041248
Received: 29 January 2020 / Revised: 10 February 2020 / Accepted: 11 February 2020 / Published: 13 February 2020
(This article belongs to the Section Molecular Biology)
NCAPG is a subunit of condensin I that plays a crucial role in chromatin condensation during mitosis. NCAPG has been demonstrated to be associated with farm animal growth traits. However, its role in regulating myoblast differentiation is still unclear. We used myoblasts derived from fetal bovine tissue as an in vitro model and found that NCAPG was expressed during myogenic differentiation in the cytoplasm and nucleus. Silencing NCAPG prolonged the mitosis and impaired the differentiation due to increased myoblast apoptosis. After 1.5 days of differentiation, silencing NCAPG enhanced muscle-specific gene expression. An assay for transposase-accessible chromatin- high throughput sequencing (ATAC-seq) revealed that silencing NCAPG altered chromatin accessibility to activating protein 1 (AP-1) and its subunits. Knocking down the expression of the AP-1 subunits fos-related antigen 2 (FOSL2) or junB proto-oncogene (JUNB) enhanced part of the muscle-specific gene expression. In conclusion, our data provide valuable evidence about NCAPG’s function in myogenesis, as well as its potential role in gene expression. View Full-Text
Keywords: NCAPG; bovine myoblasts; myogenesis; chromatin accessibility; AP-1 NCAPG; bovine myoblasts; myogenesis; chromatin accessibility; AP-1
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Hu, X.; Xing, Y.; Fu, X.; Yang, Q.; Ren, L.; Wang, Y.; Li, Q.; Li, J.; Zhang, L. NCAPG Dynamically Coordinates the Myogenesis of Fetal Bovine Tissue by Adjusting Chromatin Accessibility. Int. J. Mol. Sci. 2020, 21, 1248.

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