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Open AccessArticle

mRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules

1
Centre de Biophysique Moléculaire, UPR 4301 du CNRS, Rue Charles Sadron, 45071 Orléans, France
2
Colléguim Sciences et Techniques, Université d’Orléans, 45071 Orléans, France
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(4), 1234; https://doi.org/10.3390/ijms21041234
Received: 17 December 2019 / Revised: 4 February 2020 / Accepted: 4 February 2020 / Published: 12 February 2020
(This article belongs to the Special Issue RNA Regulatory Networks)
Stress granules and P bodies are cytoplasmic structures assembled in response to various stress factors and represent sites of temporary storage or decay of mRNAs. Depending on the source of stress, the formation of these structures may be driven by distinct mechanisms, but several stresses have been shown to stabilize mRNAs via inhibition of deadenylation. A recent study identified yeast gene deletion mutants with constitutive stress granules and elevated P bodies; however, the mechanisms which trigger its formation remain poorly understood. Here, we investigate the possibility of accumulating mRNA with mammalian codon bias, which we termed the model RNA, in these mutants. We found that the model RNA accumulates in dcp2 and xrn1 mutants and in four mutants with constitutive stress granules overlapping with P bodies. However, in eight other mutants with constitutive stress granules, the model RNA is downregulated, or its steady state levels vary. We further suggest that the accumulation of the model RNA is linked to its protection from the main mRNA surveillance path. However, there is no obvious targeting of the model RNA to stress granules or P bodies. Thus, accumulation of the model RNA and formation of constitutive stress granules occur independently and only some paths inducing formation of constitutive stress granules will stabilize mRNA as well. View Full-Text
Keywords: stress granules; Saccharomyces cerevisiae; mRNA accumulation stress granules; Saccharomyces cerevisiae; mRNA accumulation
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Kozlova, N.V.; Pichon, C.; Rahmouni, A.R. mRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules. Int. J. Mol. Sci. 2020, 21, 1234.

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