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Open AccessArticle

Estradiol-17β-Induced Changes in the Porcine Endometrial Transcriptome In Vivo

1
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland
2
Vetsuisse Faculty, Clinic of Reproductive Medicine, Genetics and Functional Genomics, University of Zurich, Eschikon 27 EHB 23.1, 8315 Lindau, Switzerland
3
District Veterinary Inspectorate in Nidzica, Limanowskiego 1, 13-100 Nidzica, Poland
4
Department of Animal Physiology and Reproduction, Institute of Biology and Biotechnology, College of Natural Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszów, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(3), 890; https://doi.org/10.3390/ijms21030890
Received: 5 December 2019 / Revised: 9 January 2020 / Accepted: 21 January 2020 / Published: 30 January 2020
(This article belongs to the Special Issue Embryo-Maternal Interactions Underlying Reproduction in Mammals)
Estradiol-17β (E2) is a key hormone regulating reproductive functions in females. In pigs, E2, as the main conceptus signal, initiates processes resulting in prolonged corpus luteum function, embryo development, and implantation. During early pregnancy the endometrium undergoes morphological and physiological transitions that are tightly related to transcriptome changes. Recently, however, the importance of E2 as a primary conceptus signal in the pig has been questionable. Thus, the aim of the present study was to determine the effects of E2 on the porcine endometrial transcriptome in vivo and to compare these effects with transcriptome profiles on day 12 of pregnancy. Microarray analysis revealed differentially expressed genes (DEGs) in response to E2 with overrepresented functional terms related to secretive functions, extracellular vesicles, cell adhesion, proliferation and differentiation, tissue rearrangements, immune response, lipid metabolism, and many others. Numerous common DEGs and processes for the endometrium on day 12 of pregnancy and E2-treated endometrium were identified. In summary, the present study is the first evidence for the effect of E2 on transcriptome profiles in porcine endometrium in vivo in the period corresponding to the maternal recognition of pregnancy. The presented results provide a valuable resource for further targeted studies considering genes and pathways regulated by conceptus-derived estrogens and their role in pregnancy establishment. View Full-Text
Keywords: estradiol-17β; early pregnancy; endometrium; transcriptome profiling; pig; Sus scrofa estradiol-17β; early pregnancy; endometrium; transcriptome profiling; pig; Sus scrofa
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Kaczynski, P.; Bauersachs, S.; Baryla, M.; Goryszewska, E.; Muszak, J.; Grzegorzewski, W.J.; Waclawik, A. Estradiol-17β-Induced Changes in the Porcine Endometrial Transcriptome In Vivo. Int. J. Mol. Sci. 2020, 21, 890.

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