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From Snapshots to Flipbook—Resolving the Dynamics of Ribosome Biogenesis with Chemical Probes
Article

Mutational Analysis of the Nsa2 N-Terminus Reveals Its Essential Role in Ribosomal 60S Subunit Assembly

Biochemie-Zentrum der Universität Heidelberg (BZH), Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
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Int. J. Mol. Sci. 2020, 21(23), 9108; https://doi.org/10.3390/ijms21239108
Received: 10 November 2020 / Revised: 23 November 2020 / Accepted: 25 November 2020 / Published: 30 November 2020
(This article belongs to the Special Issue Ribosome Biogenesis in “War and Peace of the Cell”)
The ribosome assembly factor Nsa2 is part of the Rea1-Rsa4-Nsa2 interconnected relay on nuclear pre-60S particles that is essential for 60S ribosome biogenesis. Cryo-EM structures depict Nsa2 docked via its C-terminal β-barrel domain to nuclear pre-60S particles, whereas the extended N-terminus, consisting of three α-helical segments, meanders between various 25S rRNA helices with the extreme N-terminus in close vicinity to the Nog1 GTPase center. Here, we tested whether this unappreciated proximity between Nsa2 and Nog1 is of functional importance. Our findings demonstrate that a conservative mutation, Nsa2 Q3N, abolished cell growth and impaired 60S biogenesis. Subsequent genetic and biochemical analyses verified that the Nsa2 N-terminus is required to target Nsa2 to early pre-60S particles. However, overexpression of the Nsa2 N-terminus abolished cytoplasmic recycling of the Nog1 GTPase, and both Nog1 and the Nsa2-N (1-58) construct, but not the respective Nsa2-N (1-58) Q3N mutant, were found arrested on late cytoplasmic pre-60S particles. These findings point to specific roles of the different Nsa2 domains for 60S ribosome biogenesis. View Full-Text
Keywords: ribosome assembly; ribosome biogenesis; Nsa2; Nog1; Rsa4; TINP1; Rea1; Mdn1 ribosome assembly; ribosome biogenesis; Nsa2; Nog1; Rsa4; TINP1; Rea1; Mdn1
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MDPI and ACS Style

Paternoga, H.; Früh, A.; Kunze, R.; Bradatsch, B.; Baßler, J.; Hurt, E. Mutational Analysis of the Nsa2 N-Terminus Reveals Its Essential Role in Ribosomal 60S Subunit Assembly. Int. J. Mol. Sci. 2020, 21, 9108. https://doi.org/10.3390/ijms21239108

AMA Style

Paternoga H, Früh A, Kunze R, Bradatsch B, Baßler J, Hurt E. Mutational Analysis of the Nsa2 N-Terminus Reveals Its Essential Role in Ribosomal 60S Subunit Assembly. International Journal of Molecular Sciences. 2020; 21(23):9108. https://doi.org/10.3390/ijms21239108

Chicago/Turabian Style

Paternoga, Helge, Alexander Früh, Ruth Kunze, Bettina Bradatsch, Jochen Baßler, and Ed Hurt. 2020. "Mutational Analysis of the Nsa2 N-Terminus Reveals Its Essential Role in Ribosomal 60S Subunit Assembly" International Journal of Molecular Sciences 21, no. 23: 9108. https://doi.org/10.3390/ijms21239108

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