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From Snapshots to Flipbook—Resolving the Dynamics of Ribosome Biogenesis with Chemical Probes

Institute of Molecular Biosciences, University of Graz, Humboldtstraße 50/EG, 8010 Graz, Austria
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Int. J. Mol. Sci. 2020, 21(8), 2998; https://doi.org/10.3390/ijms21082998
Received: 31 March 2020 / Revised: 17 April 2020 / Accepted: 22 April 2020 / Published: 23 April 2020
(This article belongs to the Special Issue Ribosome Biogenesis in “War and Peace of the Cell”)
The synthesis of ribosomes is one of the central and most resource demanding processes in each living cell. As ribosome biogenesis is tightly linked with the regulation of the cell cycle, perturbation of ribosome formation can trigger severe diseases, including cancer. Eukaryotic ribosome biogenesis starts in the nucleolus with pre-rRNA transcription and the initial assembly steps, continues in the nucleoplasm and is finished in the cytoplasm. From start to end, this process is highly dynamic and finished within few minutes. Despite the tremendous progress made during the last decade, the coordination of the individual maturation steps is hard to unravel by a conventional methodology. In recent years small molecular compounds were identified that specifically block either rDNA transcription or distinct steps within the maturation pathway. As these inhibitors diffuse into the cell rapidly and block their target proteins within seconds, they represent excellent tools to investigate ribosome biogenesis. Here we review how the inhibitors affect ribosome biogenesis and discuss how these effects can be interpreted by taking the complex self-regulatory mechanisms of the pathway into account. With this we want to highlight the potential of low molecular weight inhibitors to approach the dynamic nature of the ribosome biogenesis pathway. View Full-Text
Keywords: ribosome biogenesis; ribosome biogenesis inhibitors; regulation of ribosome assembly; ribosome trafficking; chemical probing; feedback regulation ribosome biogenesis; ribosome biogenesis inhibitors; regulation of ribosome assembly; ribosome trafficking; chemical probing; feedback regulation
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MDPI and ACS Style

Kofler, L.; Prattes, M.; Bergler, H. From Snapshots to Flipbook—Resolving the Dynamics of Ribosome Biogenesis with Chemical Probes. Int. J. Mol. Sci. 2020, 21, 2998. https://doi.org/10.3390/ijms21082998

AMA Style

Kofler L, Prattes M, Bergler H. From Snapshots to Flipbook—Resolving the Dynamics of Ribosome Biogenesis with Chemical Probes. International Journal of Molecular Sciences. 2020; 21(8):2998. https://doi.org/10.3390/ijms21082998

Chicago/Turabian Style

Kofler, Lisa, Michael Prattes, and Helmut Bergler. 2020. "From Snapshots to Flipbook—Resolving the Dynamics of Ribosome Biogenesis with Chemical Probes" International Journal of Molecular Sciences 21, no. 8: 2998. https://doi.org/10.3390/ijms21082998

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