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Open AccessArticle

Mass Spectrometry-Based Solid Phase Peptide Reaction Assay for Detecting Allergenicity Using an Immobilized Peptide-Conjugating Photo-Cleavable Linker

1
Medical Device Division, Innovation and Business Development Headquarters, Daicel Corporation, Minato-ku, Tokyo 108-8230, Japan
2
Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Chuo-ku, Kobe 650-0047, Japan
3
Faculty of Pharmaceutical Sciences, Sanyo-Onoda City University, Sanyo-Onoda, Yamaguchi 756-0884, Japan
4
Product Assurance Division, Mandom Corporation, Chuo-ku, Osaka 540-8530, Japan
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(21), 8332; https://doi.org/10.3390/ijms21218332
Received: 18 September 2020 / Revised: 30 October 2020 / Accepted: 30 October 2020 / Published: 6 November 2020
The biological process of skin sensitization depends on the ability of a sensitizer to modify endogenous proteins. A direct peptide reactivity assay (DPRA), based on the biological process of skin sensitization, was developed as an alternative to controversial animal experiments. Although DPRA has been endorsed by industries and is internationally accepted as promising, it has several drawbacks, such as incompatibility with hydrophobic chemicals, inability to perform detailed reaction analysis, and ability to evaluate only single components. Here, we demonstrated that sensitizers and peptide adducts can be easily identified using a mass spectrometry-based solid-phase peptide reaction assay (M-SPRA). We synthesized peptides with a photo-cleavable linker immobilized on resins. We showed the potential of M-SPRA in predicting skin sensitization by measuring the peptide adducts that were selectively eluted from the resin after cleaving the linker post-reaction. M-SPRA provides more detailed information regarding chemical reactivity and accurate assessment of test samples, including mixtures. M-SPRA may be helpful for understanding the binding mechanism of sensitizers (toxicology), which may assist in further refining reactivity assays and aiding in the interpretation of reactivity data. View Full-Text
Keywords: alternative to animal testing; immobilization; peptides; skin sensitizers; mass spectrometry; photo-labile linker alternative to animal testing; immobilization; peptides; skin sensitizers; mass spectrometry; photo-labile linker
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MDPI and ACS Style

Miyazaki, H.; Samejima, Y.; Iwata, K.; Minamino, Y.; Hikida, S.; Ariumi, H.; Ikeda, H.; Hamada, Y.; Yamashita, K.; Usui, K. Mass Spectrometry-Based Solid Phase Peptide Reaction Assay for Detecting Allergenicity Using an Immobilized Peptide-Conjugating Photo-Cleavable Linker. Int. J. Mol. Sci. 2020, 21, 8332. https://doi.org/10.3390/ijms21218332

AMA Style

Miyazaki H, Samejima Y, Iwata K, Minamino Y, Hikida S, Ariumi H, Ikeda H, Hamada Y, Yamashita K, Usui K. Mass Spectrometry-Based Solid Phase Peptide Reaction Assay for Detecting Allergenicity Using an Immobilized Peptide-Conjugating Photo-Cleavable Linker. International Journal of Molecular Sciences. 2020; 21(21):8332. https://doi.org/10.3390/ijms21218332

Chicago/Turabian Style

Miyazaki, Hiroshi; Samejima, Yasutaka; Iwata, Kazuya; Minamino, Yuuki; Hikida, Shinya; Ariumi, Hideto; Ikeda, Hidefumi; Hamada, Yoshio; Yamashita, Kunihiko; Usui, Kenji. 2020. "Mass Spectrometry-Based Solid Phase Peptide Reaction Assay for Detecting Allergenicity Using an Immobilized Peptide-Conjugating Photo-Cleavable Linker" Int. J. Mol. Sci. 21, no. 21: 8332. https://doi.org/10.3390/ijms21218332

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