Identification of a Novel Link between the Intermediate Filament Organizer IFO-1 and Cholesterol Metabolism in the Caenorhabditis elegans Intestine
Abstract
:1. Introduction
2. Results
2.1. Lipids Are Reduced in the Intestine of ifo-1 Mutants
2.2. ifo-1 Mutants Exhibit Increased Sensitivity to Cholesterol Depletion
2.3. Cholesterol Uptake and Transport Is Reduced in ifo-1 and ifb-2 Mutants
2.4. Transcriptome Profiling Identifies Striking Similarities between ifo-1 and nhr-8 Mutants
2.5. Lipidomics Reveal Common Features of ifo-1, ifb-2 and nhr-8 Mutants
2.6. nhr-8(RNAi) Mimics the Effect of Cholesterol Depletion in ifo-1(kc2) and ifb-2(kc14)
2.7. High Cholesterol Concentration Partially Rescues ifo-1(kc2) Growth Retardation
3. Discussion
4. Material and Methods
4.1. Strains
4.2. Nile Red Staining
4.3. Electron Microscopy
4.4. RNAi-Induced Larval Arrest
4.5. Cholesterol Depletion Assay
4.6. Cholesterol Uptake and Transfer Assays
4.7. Cholesterol Rescue Experiment
4.8. Gene Ontology Analysis
4.9. Lipid Extraction and Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Coch, R.A.; Geisler, F.; Annibal, A.; Antebi, A.; Leube, R.E. Identification of a Novel Link between the Intermediate Filament Organizer IFO-1 and Cholesterol Metabolism in the Caenorhabditis elegans Intestine. Int. J. Mol. Sci. 2020, 21, 8219. https://doi.org/10.3390/ijms21218219
Coch RA, Geisler F, Annibal A, Antebi A, Leube RE. Identification of a Novel Link between the Intermediate Filament Organizer IFO-1 and Cholesterol Metabolism in the Caenorhabditis elegans Intestine. International Journal of Molecular Sciences. 2020; 21(21):8219. https://doi.org/10.3390/ijms21218219
Chicago/Turabian StyleCoch, Richard A., Florian Geisler, Andrea Annibal, Adam Antebi, and Rudolf E. Leube. 2020. "Identification of a Novel Link between the Intermediate Filament Organizer IFO-1 and Cholesterol Metabolism in the Caenorhabditis elegans Intestine" International Journal of Molecular Sciences 21, no. 21: 8219. https://doi.org/10.3390/ijms21218219