Role of AT1G72910, AT1G72940, and ADR1-LIKE 2 in Plant Immunity under Nonsense-Mediated mRNA Decay-Compromised Conditions at Low Temperatures
Abstract
:1. Introduction
2. Results
2.1. The NMD-Deficient upf1 upf3 Mutants Exhibit Autoimmunity at 16 °C
2.2. Identification of AT1G72910, AT1G72940, and ADR1-LIKE 2 as NLR Genes Regulated by NMD at 16 °C
2.3. AT1G72910, AT1G72940, and ADR1-LIKE 2 Are Required for Pathogen Response at Lower Temperatures
2.4. Knockdown of AT1G72910, AT1G72940, and ADR1-LIKE 2 Can Rescue the Growth Arrest Phenotype of upf1/3 Mutants
2.5. Overexpression of AT1G72910, AT1G72940, and ADR1-LIKE 2 Results in Autoimmunity in WT Plants
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Inhibition of Translation by Cycloheximide (CHX)
4.3. Analyses of Cell Death and Pathogen Assays
4.4. RNA Sequencing
4.5. Public Transcriptome Data and Bioinformatics Analyses
4.6. qPCR Expression Analyses
4.7. Quantification of Phytohormones and Chlorophyll Contents
4.8. Determination of mRNA Degradation Kinetics
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
NLR | Nucleotide-binding, Leucine-rich Repeat |
SA | Salicylic acid |
CHX | Cycloheximide |
DEGs | Differentially expressed genes |
HR | Hypersensitive response |
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Nasim, Z.; Fahim, M.; Gawarecka, K.; Susila, H.; Jin, S.; Youn, G.; Ahn, J.H. Role of AT1G72910, AT1G72940, and ADR1-LIKE 2 in Plant Immunity under Nonsense-Mediated mRNA Decay-Compromised Conditions at Low Temperatures. Int. J. Mol. Sci. 2020, 21, 7986. https://doi.org/10.3390/ijms21217986
Nasim Z, Fahim M, Gawarecka K, Susila H, Jin S, Youn G, Ahn JH. Role of AT1G72910, AT1G72940, and ADR1-LIKE 2 in Plant Immunity under Nonsense-Mediated mRNA Decay-Compromised Conditions at Low Temperatures. International Journal of Molecular Sciences. 2020; 21(21):7986. https://doi.org/10.3390/ijms21217986
Chicago/Turabian StyleNasim, Zeeshan, Muhammad Fahim, Katarzyna Gawarecka, Hendry Susila, Suhyun Jin, Geummin Youn, and Ji Hoon Ahn. 2020. "Role of AT1G72910, AT1G72940, and ADR1-LIKE 2 in Plant Immunity under Nonsense-Mediated mRNA Decay-Compromised Conditions at Low Temperatures" International Journal of Molecular Sciences 21, no. 21: 7986. https://doi.org/10.3390/ijms21217986