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Article

Dengue Virus Infection of Aedes aegypti Alters Extracellular Vesicle Protein Cargo to Enhance Virus Transmission

1
Department of Microbiology, National Emerging Infectious Diseases Laboratories, Boston University School of Medicine, 620 Albany Street, Boston, MA 02118, USA
2
Department of Biochemistry, Boston University School of Medicine, 72 E. Concord Street, Boston, MA 02118, USA
3
Department of Entomology, Vector Biology Laboratory, Kansas State University, 1603 Old Claflin Pl, 123 Waters Hall, Manhattan, KS 66506, USA
*
Author to whom correspondence should be addressed.
Current address: Coordination of Global Environmental Changes, Ministry of Science, Technology and Innovations, Brasilia, DF 70000-000, Brazil.
Current address: Department of Entomology, Pennsylvania State University, 501 ASI Building, University Park, PA 16802, USA.
Int. J. Mol. Sci. 2020, 21(18), 6609; https://doi.org/10.3390/ijms21186609
Received: 17 August 2020 / Revised: 4 September 2020 / Accepted: 7 September 2020 / Published: 10 September 2020
(This article belongs to the Section Molecular Biology)
Dengue is the most burdensome vector-borne viral disease in the world. Dengue virus (DENV), the etiological cause of dengue, is transmitted primarily by the Aedes aegypti mosquito. Like any arbovirus, the transmission cycle of dengue involves the complex interactions of a multitude of human and mosquito factors. One point during this transmission cycle that is rich in these interactions is the biting event by the mosquito, upon which its saliva is injected into the host. A number of components in mosquito saliva have been shown to play a pivotal role in the transmission of dengue, however one such component that is not as well characterized is extracellular vesicles. Here, using high-performance liquid chromatography in tandem with mass spectrometry, we show that dengue infection altered the protein cargo of Aedes aegypti extracellular vesicles, resulting in the packaging of proteins with infection-enhancing ability. Our results support the presence of an infection-dependent pro-viral protein packaging strategy that uses the differential packaging of pro-viral proteins in extracellular vesicles of Ae. aegypti saliva to promote transmission. These studies represent the first investigation into the function of Ae. aegypti extracellular vesicle cargo during dengue infection. View Full-Text
Keywords: dengue virus; Aedes aegypti; extracellular vesicles dengue virus; Aedes aegypti; extracellular vesicles
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MDPI and ACS Style

Gold, A.S.; Feitosa-Suntheimer, F.; Araujo, R.V.; Hekman, R.M.; Asad, S.; Londono-Renteria, B.; Emili, A.; Colpitts, T.M. Dengue Virus Infection of Aedes aegypti Alters Extracellular Vesicle Protein Cargo to Enhance Virus Transmission. Int. J. Mol. Sci. 2020, 21, 6609. https://doi.org/10.3390/ijms21186609

AMA Style

Gold AS, Feitosa-Suntheimer F, Araujo RV, Hekman RM, Asad S, Londono-Renteria B, Emili A, Colpitts TM. Dengue Virus Infection of Aedes aegypti Alters Extracellular Vesicle Protein Cargo to Enhance Virus Transmission. International Journal of Molecular Sciences. 2020; 21(18):6609. https://doi.org/10.3390/ijms21186609

Chicago/Turabian Style

Gold, Alexander S., Fabiana Feitosa-Suntheimer, Ricardo V. Araujo, Ryan M. Hekman, Sultan Asad, Berlin Londono-Renteria, Andrew Emili, and Tonya M. Colpitts. 2020. "Dengue Virus Infection of Aedes aegypti Alters Extracellular Vesicle Protein Cargo to Enhance Virus Transmission" International Journal of Molecular Sciences 21, no. 18: 6609. https://doi.org/10.3390/ijms21186609

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