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Open AccessArticle

Frame Coating of Single-Walled Carbon Nanotubes in Collagen on PET Fibers for Artificial Joint Ligaments

1
Institute of Biomedical Systems, National Research University of Electronic Technology MIET, Shokin Square 1, Zelenograd, 124498 Moscow, Russia
2
Institute for Bionic Technologies and Engineering, I.M. Sechenov First Moscow State Medical University, Bolshaya Pirogovskaya street 2-4, 119991 Moscow, Russia
3
Department of Morphology and Veterinary Expertise, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, Timiryazevskaya street 49, 127550 Moscow, Russia
4
Department of Traumatology and Orthopedics, M.F. Vladimirskii Moscow Regional Research and Clinical Institute, Shepkina street 61/2, 129110 Moscow, Russia
5
Research Laboratory of Promising Processes, Scientific-Manufacturing Complex “Technological Centre”, 1-7 Shokin Square, 124498 Moscow, Russia
6
Micro- and Nanosystems Research and Development Department, Institute of Nanotechnology of Microelectronics of the Russian Academy of Sciences, 32A Leninsky Prospekt, 119991 Moscow, Russia
7
Department of Physics, Saratov State University, Astrakhanskaya street 83, 410012 Saratov, Russia
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(17), 6163; https://doi.org/10.3390/ijms21176163
Received: 15 July 2020 / Revised: 18 August 2020 / Accepted: 20 August 2020 / Published: 26 August 2020
(This article belongs to the Special Issue Nanomaterials for Tissue Engineering Applications)
The coating formation technique for artificial knee ligaments was proposed, which provided tight fixation of ligaments of polyethylene terephthalate (PET) fibers as a result of the healing of the bone channel in the short-term period after implantation. The coating is a frame structure of single-walled carbon nanotubes (SWCNT) in a collagen matrix, which is formed by layer-by-layer solidification of an aqueous dispersion of SWCNT with collagen during spin coating and controlled irradiation with IR radiation. Quantum mechanical method SCC DFTB, with a self-consistent charge, was used. It is based on the density functional theory and the tight-binding approximation. The method established the optimal temperature and time for the formation of the equilibrium configurations of the SWCNT/collagen type II complexes to ensure maximum binding energies between the nanotube and the collagen. The highest binding energies were observed in complexes with SWCNT nanometer diameter in comparison with subnanometer SWCNT. The coating had a porous structure—pore size was 0.5—6 μm. The process of reducing the mass and volume of the coating with the initial biodegradation of collagen after contact with blood plasma was demonstrated. This is proved by exceeding the intensity of the SWCNT peaks G and D after contact with the blood serum in the Raman spectrum and by decreasing the intensity of the main collagen bands in the SWCNT/collagen complex frame coating. The number of pores and their size increased to 20 μm. The modification of the PET tape with the SWCNT/collagen coating allowed to increase its hydrophilicity by 1.7 times compared to the original PET fibers and by 1.3 times compared to the collagen coating. A reduced hemolysis level of the PET tape coated with SWCNT/collagen was achieved. The SWCNT/collagen coating provided 2.2 times less hemolysis than an uncoated PET implant. MicroCT showed the effective formation of new bone and dense connective tissue around the implant. A decrease in channel diameter from 2.5 to 1.7 mm was detected at three and, especially, six months after implantation of a PET tape with SWCNT/collagen coating. MicroCT allowed us to identify areas for histological sections, which demonstrated the favorable interaction of the PET tape with the surrounding tissues. In the case of using the PET tape coated with SWCNT/collagen, more active growth of connective tissue with mature collagen fibers in the area of implantation was observed than in the case of only collagen coating. The stimulating effect of SWCNT/collagen on the formation of bone trabeculae around and inside the PET tape was evident in three and six months after implantation. Thus, a PET tape with SWCNT/collagen coating has osteoconductivity as well as a high level of hydrophilicity and hemocompatibility. View Full-Text
Keywords: nanostructured materials; coating; single-walled carbon nanotubes; collagen; IR radiation; PET fibers; artificial knee ligaments; bone regeneration; biodegradation; osteoconductivity; hemocompatibility nanostructured materials; coating; single-walled carbon nanotubes; collagen; IR radiation; PET fibers; artificial knee ligaments; bone regeneration; biodegradation; osteoconductivity; hemocompatibility
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MDPI and ACS Style

Gerasimenko, A.Y.; Zhurbina, N.N.; Cherepanova, N.G.; Semak, A.E.; Zar, V.V.; Fedorova, Y.O.; Eganova, E.M.; Pavlov, A.A.; Telyshev, D.V.; Selishchev, S.V.; Glukhova, O.E. Frame Coating of Single-Walled Carbon Nanotubes in Collagen on PET Fibers for Artificial Joint Ligaments. Int. J. Mol. Sci. 2020, 21, 6163. https://doi.org/10.3390/ijms21176163

AMA Style

Gerasimenko AY, Zhurbina NN, Cherepanova NG, Semak AE, Zar VV, Fedorova YO, Eganova EM, Pavlov AA, Telyshev DV, Selishchev SV, Glukhova OE. Frame Coating of Single-Walled Carbon Nanotubes in Collagen on PET Fibers for Artificial Joint Ligaments. International Journal of Molecular Sciences. 2020; 21(17):6163. https://doi.org/10.3390/ijms21176163

Chicago/Turabian Style

Gerasimenko, Alexander Y.; Zhurbina, Natalia N.; Cherepanova, Nadezhda G.; Semak, Anna E.; Zar, Vadim V.; Fedorova, Yulia O.; Eganova, Elena M.; Pavlov, Alexander A.; Telyshev, Dmitry V.; Selishchev, Sergey V.; Glukhova, Olga E. 2020. "Frame Coating of Single-Walled Carbon Nanotubes in Collagen on PET Fibers for Artificial Joint Ligaments" Int. J. Mol. Sci. 21, no. 17: 6163. https://doi.org/10.3390/ijms21176163

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