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Article

Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro

1
Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland
2
Department of Experimental Neuroendocrinology, Maj Institute of Pharmacology, Polish Academy of Sciences, 31-343 Krakow, Poland
3
Institute of Nuclear Physics, Polish Academy of Sciences, 31-342 Krakow, Poland
4
Department of Oral Surgery, Faculty of Medicine, Jagiellonian University Medical College, 31-155 Krakow, Poland
5
Laboratory of Stem Cell Biotechnology, Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland
6
Polish Stem Cell Bank, 00-867 Warsaw, Poland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(17), 6172; https://doi.org/10.3390/ijms21176172
Received: 28 July 2020 / Revised: 20 August 2020 / Accepted: 25 August 2020 / Published: 26 August 2020
(This article belongs to the Special Issue Cells and Materials for Disease Modeling and Regenerative Medicine)
Human dental pulp harbours unique stem cell population exhibiting mesenchymal stem/stromal cell (MSC) characteristics. This study aimed to analyse the differentiation potential and other essential functional and morphological features of dental pulp stem cells (DPSCs) in comparison with Wharton’s jelly-derived MSCs from the umbilical cord (UC-MSCs), and to evaluate the osteogenic differentiation of DPSCs in 3D culture with a hypoxic microenvironment resembling the stem cell niche. Human DPSCs as well as UC-MSCs were isolated from primary human tissues and were subjected to a series of experiments. We established a multiantigenic profile of DPSCs with CD45/CD14/CD34/CD29+/CD44+/CD73+/CD90+/CD105+/Stro-1+/HLA-DR (using flow cytometry) and confirmed their tri-lineage osteogenic, chondrogenic, and adipogenic differentiation potential (using qRT-PCR and histochemical staining) in comparison with the UC-MSCs. The results also demonstrated the potency of DPSCs to differentiate into osteoblasts in vitro. Moreover, we showed that the DPSCs exhibit limited cardiomyogenic and endothelial differentiation potential. Decreased proliferation and metabolic activity as well as increased osteogenic differentiation of DPSCs in vitro, attributed to 3D cell encapsulation and low oxygen concentration, were also observed. DPSCs exhibiting elevated osteogenic potential may serve as potential candidates for a cell-based product for advanced therapy, particularly for bone repair. Novel tissue engineering approaches combining DPSCs, 3D biomaterial scaffolds, and other stimulating chemical factors may represent innovative strategies for pro-regenerative therapies. View Full-Text
Keywords: stem cells; dental pulp stem cells; osteogenesis; biomaterials; tissue engineering; regenerative medicine stem cells; dental pulp stem cells; osteogenesis; biomaterials; tissue engineering; regenerative medicine
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MDPI and ACS Style

Labedz-Maslowska, A.; Bryniarska, N.; Kubiak, A.; Kaczmarzyk, T.; Sekula-Stryjewska, M.; Noga, S.; Boruczkowski, D.; Madeja, Z.; Zuba-Surma, E. Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro. Int. J. Mol. Sci. 2020, 21, 6172. https://doi.org/10.3390/ijms21176172

AMA Style

Labedz-Maslowska A, Bryniarska N, Kubiak A, Kaczmarzyk T, Sekula-Stryjewska M, Noga S, Boruczkowski D, Madeja Z, Zuba-Surma E. Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro. International Journal of Molecular Sciences. 2020; 21(17):6172. https://doi.org/10.3390/ijms21176172

Chicago/Turabian Style

Labedz-Maslowska, Anna, Natalia Bryniarska, Andrzej Kubiak, Tomasz Kaczmarzyk, Malgorzata Sekula-Stryjewska, Sylwia Noga, Dariusz Boruczkowski, Zbigniew Madeja, and Ewa Zuba-Surma. 2020. "Multilineage Differentiation Potential of Human Dental Pulp Stem Cells—Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro" International Journal of Molecular Sciences 21, no. 17: 6172. https://doi.org/10.3390/ijms21176172

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