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Lipidomic Biomarkers in Polycystic Ovary Syndrome and Endometrial Cancer
Open AccessArticle

D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome

1
Departamento de Reproducción Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain
2
Laboratorio de Neuropsicofarmacología, Unidad de Gestión Clínica de Salud Mental, Instituto IBIMA, Hospital Regional Universitario de Málaga, 29010 Málaga, Spain
3
Euronutra S.L., Calle Johannes Kepler 3, 29590 Málaga, Spain
4
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Málaga, 29010 Málaga, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(17), 6049; https://doi.org/10.3390/ijms21176049
Received: 10 July 2020 / Revised: 17 August 2020 / Accepted: 20 August 2020 / Published: 22 August 2020
(This article belongs to the Special Issue Polycystic Ovary Syndrome: From Molecular Mechanisms to Therapies)
Polycystic ovarian syndrome (PCOS) is the main cause of female infertility. It is a multifactorial disorder with varying clinical manifestations including metabolic/endocrine abnormalities, hyperandrogenism, and ovarian cysts, among other conditions. D-Chiro-inositol (DCI) is the main treatment available for PCOS in humans. To address some of the mechanisms of this complex disorder and its treatment, this study examines the effect of DCI on reproduction during the development of different PCOS-associated phenotypes in aged females and two mouse models of PCOS. Aged females (8 months old) were treated or not (control) with DCI for 2 months. PCOS models were generated by treatment with dihydrotestosterone (DHT) on Days 16, 17, and 18 of gestation, or by testosterone propionate (TP) treatment on the first day of life. At two months of age, PCOS mice were treated with DCI for 2 months and their reproductive parameters analyzed. No effects of DCI treatment were produced on body weight or ovary/body weight ratio. However, treatment reduced the number of follicles with an atretic cyst-like appearance and improved embryo development in the PCOS models, and also increased implantation rates in both aged and PCOS mice. DCI modified the expression of genes related to oocyte quality, oxidative stress, and luteal sufficiency in cumulus-oocyte complexes (COCs) obtained from the aged and PCOS models. Further, the phosphorylation of AKT, a main metabolic sensor activated by insulin in the liver, was enhanced only in the DHT group, which was the only PCOS model showing glucose intolerance and AKT dephosphorylation. The effect of DCI in the TP model seemed mediated by its influence on oxidative stress and follicle insufficiency. Our results indicate that DCI works in preclinical models of PCOS and offer insight into its mechanism of action when used to treat this infertility-associated syndrome. View Full-Text
Keywords: polycystic ovary syndrome; D-chiro-inositol; oocyte; embryo; fertility; oxidative stress; glucose intolerance polycystic ovary syndrome; D-chiro-inositol; oocyte; embryo; fertility; oxidative stress; glucose intolerance
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MDPI and ACS Style

Pericuesta, E.; Laguna-Barraza, R.; Ramos-Ibeas, P.; Gutierrez-Arroyo, J.L.; Navarro, J.A.; Vera, K.; Sanjuan, C.; Baixeras, E.; de Fonseca, F.R.; Gutierrez-Adan, A. D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome. Int. J. Mol. Sci. 2020, 21, 6049. https://doi.org/10.3390/ijms21176049

AMA Style

Pericuesta E, Laguna-Barraza R, Ramos-Ibeas P, Gutierrez-Arroyo JL, Navarro JA, Vera K, Sanjuan C, Baixeras E, de Fonseca FR, Gutierrez-Adan A. D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome. International Journal of Molecular Sciences. 2020; 21(17):6049. https://doi.org/10.3390/ijms21176049

Chicago/Turabian Style

Pericuesta, Eva; Laguna-Barraza, Ricardo; Ramos-Ibeas, Priscila; Gutierrez-Arroyo, Julia L.; Navarro, Juan A.; Vera, Katia; Sanjuan, Carlos; Baixeras, Elena; de Fonseca, Fernando R.; Gutierrez-Adan, Alfonso. 2020. "D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome" Int. J. Mol. Sci. 21, no. 17: 6049. https://doi.org/10.3390/ijms21176049

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