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Article

Lactobacillus Strains Alleviated Hyperlipidemia and Liver Steatosis in Aging Rats via Activation of AMPK

1
School of Industrial Technology, Universiti Sains Malaysia, Penang 11800, Malaysia
2
USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, Penang 11800, Malaysia
3
Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
4
School of Biological Science, Universiti Sains Malaysia, Penang 11800, Malaysia
5
School of Data Sciences, Perdana University, MARDI Complex, Selangor 43400, Malaysia
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(16), 5872; https://doi.org/10.3390/ijms21165872
Received: 8 June 2020 / Revised: 1 July 2020 / Accepted: 1 July 2020 / Published: 16 August 2020
(This article belongs to the Special Issue Nutraceuticals in Chronic Diseases)
In this study, we hypothesized that different strains of Lactobacillus can alleviate hyperlipidemia and liver steatosis via activation of 5′ adenosine monophosphate-activated protein kinase (AMPK), an enzyme that is involved in cellular energy homeostasis, in aged rats. Male rats were fed with a high-fat diet (HFD) and injected with D-galactose daily over 12 weeks to induce aging. Treatments included (n = 6) (i) normal diet (ND), (ii) HFD, (iii) HFD-statin (lovastatin 2 mg/kg/day), (iv) HFD-Lactobacillus fermentum DR9 (10 log CFU/day), (v) HFD-Lactobacillus plantarum DR7 (10 log CFU/day), and (vi) HFD-Lactobacillus reuteri 8513d (10 log CFU/day). Rats administered with statin, DR9, and 8513d reduced serum total cholesterol levels after eight weeks (p < 0.05), while the administration of DR7 reduced serum triglycerides level after 12 weeks (p < 0.05) as compared to the HFD control. A more prominent effect was observed from the administration of DR7, where positive effects were observed, ranging from hepatic gene expressions to liver histology as compared to the control (p < 0.05); downregulation of hepatic lipid synthesis and β-oxidation gene stearoyl-CoA desaturase 1 (SCD1), upregulation of hepatic sterol excretion genes of ATP-binding cassette subfamily G member 5 and 8 (ABCG5 and ABCG8), lesser degree of liver steatosis, and upregulation of hepatic energy metabolisms genes AMPKα1 and AMPKα2. Taken altogether, this study illustrated that the administration of selected Lactobacillus strains led to improved lipid profiles via activation of energy and lipid metabolisms, suggesting the potentials of Lactobacillus as a promising natural intervention for alleviation of cardiovascular and liver diseases. View Full-Text
Keywords: triglyceride; immune; AMPK; Lactobacillus; NAFLD; SCD1 triglyceride; immune; AMPK; Lactobacillus; NAFLD; SCD1
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MDPI and ACS Style

Lew, L.-C.; Hor, Y.-Y.; Jaafar, M.-H.; Lau, A.-S.-Y.; Lee, B.-K.; Chuah, L.-O.; Yap, K.-P.; Azlan, A.; Azzam, G.; Choi, S.-B.; Liong, M.-T. Lactobacillus Strains Alleviated Hyperlipidemia and Liver Steatosis in Aging Rats via Activation of AMPK. Int. J. Mol. Sci. 2020, 21, 5872. https://doi.org/10.3390/ijms21165872

AMA Style

Lew L-C, Hor Y-Y, Jaafar M-H, Lau A-S-Y, Lee B-K, Chuah L-O, Yap K-P, Azlan A, Azzam G, Choi S-B, Liong M-T. Lactobacillus Strains Alleviated Hyperlipidemia and Liver Steatosis in Aging Rats via Activation of AMPK. International Journal of Molecular Sciences. 2020; 21(16):5872. https://doi.org/10.3390/ijms21165872

Chicago/Turabian Style

Lew, Lee-Ching, Yan-Yan Hor, Mohamad-Hafis Jaafar, Amy-Sie-Yik Lau, Boon-Kiat Lee, Li-Oon Chuah, Kien-Pong Yap, Azali Azlan, Ghows Azzam, Sy-Bing Choi, and Min-Tze Liong. 2020. "Lactobacillus Strains Alleviated Hyperlipidemia and Liver Steatosis in Aging Rats via Activation of AMPK" International Journal of Molecular Sciences 21, no. 16: 5872. https://doi.org/10.3390/ijms21165872

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