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Vascular Remodeling in Moyamoya Angiopathy: From Peripheral Blood Mononuclear Cells to Endothelial Cells

1
Laboratory of Cellular Neurobiology, Neurology IX Unit, UCV, Fondazione IRCCS Istituto Neurologico Carlo Besta, 20133 Milan, Italy
2
Immunology and Cell Therapy Unit, Tettamanti Research Center, University of Milano-Bicocca, 20900 Monza, Italy
3
Neurology IX Unit, UCV, Fondazione IRCCS Istituto Neurologico Carlo Besta, 20133 Milan, Italy
4
Diagnostic Imaging & Interventional Neuroradiology Unit, Fondazione IRCCS Istituto Neurologico Carlo Besta, 20133 Milan, Italy
5
Neurosurgical II Unit, Fondazione IRCCS Istituto Neurologico Carlo Besta, 20133 Milan, Italy
6
Developmental Neurology Unit, Fondazione IRCCS Istituto Neurologico Carlo Besta, 20133 Milan, Italy
7
Department of Pediatric Neuroscience, Fondazione IRCCS Istituto Neurologico Carlo Besta, 20133 Milan, Italy
8
Neurology Unit, Stroke Unit, Azienda Unità Sanitaria Locale—IRCCS di Reggio Emilia, 42122 Reggio Emilia, Italy
9
Department of Clinical and Experimental Sciences, Clinica Neurologica, Università degli Studi di Brescia, 25121 Brescia, Italy
10
Unit of Neurology, Neurophysiology, Neurobiology, Department of Medicine, Università Campus Bio-Medico di Roma, 00128 Rome, Italy
11
Stroke Unit, Neurology Clinic, Department of Biomedical Metabolic and Neural Sciences, Nuovo Ospedale Civile S Agostino Estense, University of Modena and Reggio Emilia, 41126 Modena, Italy
12
Department of Neurosurgery, Charite Universitätsmedizin, 10117 Berlin, Germany
13
Department of Genetics, Lariboisière Hospital and INSERM U1141, Paris-Diderot University, 75010 Paris, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(16), 5763; https://doi.org/10.3390/ijms21165763
Received: 2 July 2020 / Revised: 30 July 2020 / Accepted: 6 August 2020 / Published: 11 August 2020
(This article belongs to the Special Issue Cell Signaling in Neurodegeneration)
The pathophysiological mechanisms of Moyamoya angiopathy (MA), which is a rare cerebrovascular condition characterized by recurrent ischemic/hemorrhagic strokes, are still largely unknown. An imbalance of vasculogenic/angiogenic mechanisms has been proposed as one possible disease aspect. Circulating endothelial progenitor cells (cEPCs) have been hypothesized to contribute to vascular remodeling of MA, but it remains unclear whether they might be considered a disease effect or have a role in disease pathogenesis. The aim of the present study was to provide a morphological, phenotypical, and functional characterization of the cEPCs from MA patients to uncover their role in the disease pathophysiology. cEPCs were identified from whole blood as CD45dimCD34+CD133+ mononuclear cells. Morphological, biochemical, and functional assays were performed to characterize cEPCs. A significant reduced level of cEPCs was found in blood samples collected from a homogeneous group of adult (mean age 46.86 ± 11.7; 86.36% females), Caucasian, non-operated MA patients with respect to healthy donors (HD; p = 0.032). Since no difference in cEPC characteristics and functionality was observed between MA patients and HD, a defective recruitment mechanism could be involved in the disease pathophysiology. Collectively, our results suggest that cEPC level more than endothelial progenitor cell (EPC) functionality seems to be a potential marker of MA. The validation of our results on a larger population and the correlation with clinical data as well as the use of more complex cellular model could help our understanding of EPC role in MA pathophysiology. View Full-Text
Keywords: neovascularization; Moyamoya angiopathy; endothelial progenitor cells; RNF213 neovascularization; Moyamoya angiopathy; endothelial progenitor cells; RNF213
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Tinelli, F.; Nava, S.; Arioli, F.; Bedini, G.; Scelzo, E.; Lisini, D.; Faragò, G.; Gioppo, A.; Ciceri, E.F.; Acerbi, F.; Ferroli, P.; Vetrano, I.G.; Esposito, S.; Saletti, V.; Pantaleoni, C.; Zibordi, F.; Nardocci, N.; Zedde, M.L.; Pezzini, A.; Di Lazzaro, V.; Capone, F.; Dell’Acqua, M.L.; Vajkoczy, P.; Tournier-Lasserve, E.; Parati, E.A.; Bersano, A.; Gatti, L. Vascular Remodeling in Moyamoya Angiopathy: From Peripheral Blood Mononuclear Cells to Endothelial Cells. Int. J. Mol. Sci. 2020, 21, 5763.

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