Decreased Expression of ZNF554 in Gliomas is Associated with the Activation of Tumor Pathways and Shorter Patient Survival
Abstract
:1. Introduction
2. Results
2.1. Brain Tissue Expression Pattern of ZNF554
2.2. ZNF554 Protein Expression in Gliomas
2.3. ZNF554 mRNA Expression in Gliomas
2.4. The Effect of ZNF554 Overexpression in Glioblastoma Cells
3. Discussion
3.1. Principal Findings of This Study:
3.2. ZNFs in the Normal Human Brain
3.3. ZNFs are Double-Edged Swords in Different Cancer Types
3.4. ZNF554 as a Potential Tumor Suppressor in Gliomas
3.5. Potential Diagnostic and Therapeutic Implications of ZNF554 in Gliomas
3.6. Strengths and Limitations
4. Conclusions
5. Materials and Methods
5.1. Analysis of ZNF554 mRNA Expression in Normal Brain
5.2. Analysis of ZNF554 Protein Expression in Gliomas
5.3. Immunohistochemistry for ZNF554
5.4. Evaluation of ZNF554 Immunostainings
5.5. Analysis of ZNF554 mRNA Expression and Patients’ Survival in Gliomas
5.6. Transient Transfection of U87 Glioblastoma Cells with ZNF554
5.7. Total RNA Isolation
5.8. Labeling and Microarray Hybridization
5.9. Quantitative Real-Time PCR
5.10. Proliferation/Viability Assay
5.11. Cell Cycle Assay
5.12. Data Analysis
5.12.1. Allen Brain Atlas Data Analysis
5.12.2. TCGA RNA-Seq Data Analysis
5.12.3. Microarray Data Analysis of Transfected U87 Cells
5.12.4. Pathway Analysis
5.12.5. Quantitative RT-PCR Data Analysis
5.12.6. Data Analysis of ZNF554 Immunostainings
5.12.7. Data Analysis of Cell Proliferation/Viability and Cell Cycle
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Groups | Grade | na | Age (Years) at Diagnosis a | Male/Female b |
---|---|---|---|---|
Control (epilepsy) | - | 11 | 38.0 (14–64) | 5/6 |
Oligodendroglioma | II | 10 | 51.5 (29–65) | 3/7 |
Anaplastic oligondendroglioma | III | 10 | 47.0 (30–67) | 3/7 |
Diffuse astrocytoma | II | 10 | 47.0 (29–69) | 4/6 |
Anaplastic astrocytoma | III | 10 | 41.5 (31–78) | 6/4 |
Glioblastoma multiforme | IV | 11 | 64.0 (24–73) | 6/5 |
Gene Symbol | Gene Name | Assay ID |
---|---|---|
ZNF554 | Zinc finger protein 554 | Hs00171072_m1 |
IL6 | Interleukin 6 | Hs00174131_m1 |
IGFBP3 | Insulin-like growth factor-binding protein 3 | Hs00181211_m1 |
RPLP0 | Ribosomal protein lateral stalk subunit P0 | Hs99999902_m1 |
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Balogh, A.; Reiniger, L.; Hetey, S.; Kiraly, P.; Toth, E.; Karaszi, K.; Juhasz, K.; Gelencser, Z.; Zvara, A.; Szilagyi, A.; et al. Decreased Expression of ZNF554 in Gliomas is Associated with the Activation of Tumor Pathways and Shorter Patient Survival. Int. J. Mol. Sci. 2020, 21, 5762. https://doi.org/10.3390/ijms21165762
Balogh A, Reiniger L, Hetey S, Kiraly P, Toth E, Karaszi K, Juhasz K, Gelencser Z, Zvara A, Szilagyi A, et al. Decreased Expression of ZNF554 in Gliomas is Associated with the Activation of Tumor Pathways and Shorter Patient Survival. International Journal of Molecular Sciences. 2020; 21(16):5762. https://doi.org/10.3390/ijms21165762
Chicago/Turabian StyleBalogh, Andrea, Lilla Reiniger, Szabolcs Hetey, Peter Kiraly, Eszter Toth, Katalin Karaszi, Kata Juhasz, Zsolt Gelencser, Agnes Zvara, Andras Szilagyi, and et al. 2020. "Decreased Expression of ZNF554 in Gliomas is Associated with the Activation of Tumor Pathways and Shorter Patient Survival" International Journal of Molecular Sciences 21, no. 16: 5762. https://doi.org/10.3390/ijms21165762