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Article

Time-Dependent miRNA Profile during Septic Acute Kidney Injury in Mice

1
Institute of Translational Medicine, Semmelweis University, 1094 Budapest, Hungary
2
Institute for Translational Medicine, Medical School, University of Pécs, 7624 Pécs, Hungary
3
Department of Biochemistry and Molecular and Structural Biology, Jožef Stefan Institute, 1000 Ljubljana, Slovenia
4
Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, 1000 Ljubljana, Slovenia
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(15), 5316; https://doi.org/10.3390/ijms21155316
Received: 13 July 2020 / Accepted: 25 July 2020 / Published: 27 July 2020
(This article belongs to the Special Issue Inflammation and Oxidative Stress in Kidney Disease)
(1) Background: Lipopolysaccharide (LPS)-induced systemic inflammation is associated with septic acute kidney injury (AKI). We investigated the time-dependent miRNA expression changes in the kidney caused by LPS. (2) Methods: Male outbred NMRI mice were injected with LPS and sacrificed at 1.5 and 6 h (40 mg/kg i.p., early phase, EP) or at 24 and 48 h (10 mg/kg i.p., late phase, LP). The miRNA profile was established using miRCURY LNA™ microarray and confirmed with qPCR. Total renal proteome was analyzed by LC-MS/MS (ProteomeXchange: PXD014664). (3) Results: Septic AKI was confirmed by increases in plasma urea concentration and in renal TNF-α and IL-6 mRNA expression. Most miRNAs were altered at 6 and 24 h and declined by 48 h. In EP miR-762 was newly identified and validated and was the most elevated miRNA. The predicted target of miR-762, Ras related GTPase 1B (Sar1b) was downregulated. In LP miR-21a-5p was the most influenced miRNA followed by miR-451a, miR-144-3p, and miR-146a-5p. Among the potential protein targets of the most influenced miRNAs, only aquaporin-1, a target of miR-144-3p was downregulated at 24 h. (4) Conclusion: Besides already known miRNAs, septic AKI upregulated miR-762, which may regulate GTP signaling, and miR-144-3p and downregulated its target, aquaporin-1. View Full-Text
Keywords: septic acute kidney injury; miArray; miR-21a; miR-144-3p; miR-146a-5p; miR-451a; miR-762; aquaporin-1; cross-tolerance septic acute kidney injury; miArray; miR-21a; miR-144-3p; miR-146a-5p; miR-451a; miR-762; aquaporin-1; cross-tolerance
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MDPI and ACS Style

Tod, P.; Róka, B.; Kaucsár, T.; Szatmári, K.; Vizovišek, M.; Vidmar, R.; Fonovič, M.; Szénási, G.; Hamar, P. Time-Dependent miRNA Profile during Septic Acute Kidney Injury in Mice. Int. J. Mol. Sci. 2020, 21, 5316. https://doi.org/10.3390/ijms21155316

AMA Style

Tod P, Róka B, Kaucsár T, Szatmári K, Vizovišek M, Vidmar R, Fonovič M, Szénási G, Hamar P. Time-Dependent miRNA Profile during Septic Acute Kidney Injury in Mice. International Journal of Molecular Sciences. 2020; 21(15):5316. https://doi.org/10.3390/ijms21155316

Chicago/Turabian Style

Tod, Pál, Beáta Róka, Tamás Kaucsár, Krisztina Szatmári, Matej Vizovišek, Robert Vidmar, Marko Fonovič, Gábor Szénási, and Péter Hamar. 2020. "Time-Dependent miRNA Profile during Septic Acute Kidney Injury in Mice" International Journal of Molecular Sciences 21, no. 15: 5316. https://doi.org/10.3390/ijms21155316

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